Multipoint immobilization and stabilization of amined peroxidases from soybean hull and chayote employing bacterial cellulose as support

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorBiopolymers and Biomaterials Laboratory-
Autor(es): dc.contributorFacultad de Ciencias Químicas Guayaquil-
Autor(es): dc.contributorFacultad de Ciencias Exactas y Naturales-
Autor(es): dc.creatorVinueza Galárraga, Julio César [UNESP]-
Autor(es): dc.creatorda Silva Barud, Hernane-
Autor(es): dc.creatorDos Santos, Fernanda Kolenyak-
Autor(es): dc.creatorDavanso, Maisa [UNESP]-
Autor(es): dc.creatorPaz-Cedeño, Fernando Roberto [UNESP]-
Autor(es): dc.creatorde Paula, Ariela Veloso [UNESP]-
Autor(es): dc.creatorMonti, Rubens [UNESP]-
Autor(es): dc.creatorMasarin, Fernando [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:34:32Z-
Data de disponibilização: dc.date.available2022-02-22T00:34:32Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.35812/CELLULOSECHEMTECHNOL.2020.54.29-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201795-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201795-
Descrição: dc.descriptionPeroxidases are homoproteins that catalyze redox processes, generating free radicals and polyaromatic products insoluble in water, facilitating their removal from the aqueous medium. The objective of this research was to extract the peroxidases of soybean and chayote, immobilize these enzymes on a highly activated bacterial cellulose (BC) support and use the derivatives obtained for discoloration of bromophenol blue. The amination of soluble peroxidases was realized in ethylenediamine buffer, at pH 4.75, and 10 and 50 mM carbodiimide. Amined peroxidases of 10 and 50 mM were covalently immobilized on the BC-glyoxyl support, with recovered activity of 82% for the derivative BC-Gly-S-NH2 50 mM and 92% for the derivative BC-Gly-Ch-NH2 50 mM. Total discoloration of bromophenol blue was obtained in 60 min, using the four amino derivatives. The derivatives were reused for five cycles and they maintained an average of 40% of their catalytic properties, suggesting that these products are suitable as a low-cost alternative for wastewater treatment and other industrial processes.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences (FCF) Department of Bioprocess Engineering and Biotechnology-
Descrição: dc.descriptionAraraquara University Center (UNIARA) Biopolymers and Biomaterials Laboratory-
Descrição: dc.descriptionUniversidad de Guayaquil (UG) Facultad de Ciencias Químicas Guayaquil-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry (IQ)-
Descrição: dc.descriptionEcuador Pontificia Universidad Católica del Ecuador Facultad de Ciencias Exactas y Naturales-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences (FCF) Department of Bioprocess Engineering and Biotechnology-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry (IQ)-
Descrição: dc.descriptionFAPESP: 2014/12563-2-
Formato: dc.format275-283-
Idioma: dc.languageen-
Relação: dc.relationCellulose Chemistry and Technology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBacterial cellulose-
Palavras-chave: dc.subjectChayote-
Palavras-chave: dc.subjectDiscoloration of the bromophenol dye-
Palavras-chave: dc.subjectImmobilization-
Palavras-chave: dc.subjectPeroxidases extraction-
Palavras-chave: dc.subjectSoybean hull-
Título: dc.titleMultipoint immobilization and stabilization of amined peroxidases from soybean hull and chayote employing bacterial cellulose as support-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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