Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Brown University | - |
Autor(es): dc.creator | de Camargo Ribeiro, Felipe [UNESP] | - |
Autor(es): dc.creator | Junqueira, Juliana Campos [UNESP] | - |
Autor(es): dc.creator | dos Santos, Jéssica Diane [UNESP] | - |
Autor(es): dc.creator | de Barros, Patrícia Pimentel [UNESP] | - |
Autor(es): dc.creator | Rossoni, Rodnei Dennis [UNESP] | - |
Autor(es): dc.creator | Shukla, Shashank | - |
Autor(es): dc.creator | Fuchs, Beth Burgwyn | - |
Autor(es): dc.creator | Shukla, Anita | - |
Autor(es): dc.creator | Mylonakis, Eleftherios | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:34:32Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:34:32Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-06-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1128/AAC.02323-19 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/201794 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/201794 | - |
Descrição: dc.description | Probiotics might provide an alternative approach for the control of oral candidiasis. However, studies on the antifungal activity of probiotics in the oral cavity are based on the consumption of yogurt or other dietary products, and it is necessary to use appropriate biomaterials and specific strains to obtain probiotic formulations targeted for local oral administration. In this study, we impregnated gellan gum, a natural biopolymer used as a food additive, with a probiotic and investigated its antifungal activity against Candida albicans. Lactobacillus paracasei 28.4, a strain recently isolated from the oral cavity of a caries-free individual, was incorporated in several concentrations of gellan gum (0.6% to 1% [wt/vol]). All tested concentrations could incorporate L. paracasei cells while maintaining bacterial viability. Probiotic-gellan gum formulations were stable for 7 days when stored at room temperature or 4°C. Long-term storage of bacterium-impregnated gellan gum was achieved when L. paracasei 28.4 was lyophilized. The probiotic-gellan gum formulations provided a release of L. paracasei cells over 24 h that was sufficient to inhibit the growth of C. albicans, with effects dependent on the cell concentrations incorporated into gellan gum. The probiotic-gellan gum formulations also had inhibitory activity against Candida sp. biofilms by reducing the number of Candida sp. cells (P < 0.0001), decreasing the total biomass (P = 0.0003), and impairing hyphae formation (P = 0.0002), compared to the control group which received no treatment. Interestingly, a probiotic formulation of 1% (wt/vol) gellan gum provided an oral colonization of L. paracasei in mice with approximately 6 log CFU/ml after 10 days. This formulation inhibited C. albicans growth (P < 0.0001), prevented the development of candidiasis lesions (P = 0.0013), and suppressed inflammation (P = 0.0006) compared to the mice not treated in the microscopic analysis of the tongue dorsum. These results indicate that gellan gum is a promising biomaterial and can be used as a carrier system to promote oral colonization for probiotics that prevent oral candidiasis. | - |
Descrição: dc.description | National Institutes of Health | - |
Descrição: dc.description | Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University/UNESP | - |
Descrição: dc.description | School of Engineering Brown University | - |
Descrição: dc.description | Division of Infectious Disease Alpert Medical School Brown University | - |
Descrição: dc.description | Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University/UNESP | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Antimicrobial Agents and Chemotherapy | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Candida albicans | - |
Palavras-chave: dc.subject | Gellan gum | - |
Palavras-chave: dc.subject | Hydrogels | - |
Palavras-chave: dc.subject | Lactobacillus paracasei | - |
Palavras-chave: dc.subject | Oral candidiasis | - |
Título: dc.title | Development of probiotic formulations for oral candidiasis prevention: Gellan Gum as a Carrier to Deliver Lactobacillus paracasei 28.4 | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: