Mitochondrial Respiratory Inhibition Promoted by Pyraclostrobin in Fungi is Also Observed in Honey Bees

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorNicodemo, Daniel [UNESP]-
Autor(es): dc.creatorMingatto, Fábio Erminio [UNESP]-
Autor(es): dc.creatorDe Jong, David-
Autor(es): dc.creatorBizerra, Paulo Francisco Veiga [UNESP]-
Autor(es): dc.creatorTavares, Marco Aurélio [UNESP]-
Autor(es): dc.creatorBellini, William Cesar [UNESP]-
Autor(es): dc.creatorVicente, Eduardo Festozo [UNESP]-
Autor(es): dc.creatorde Carvalho, Amanda [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:34:31Z-
Data de disponibilização: dc.date.available2022-02-22T00:34:31Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/etc.4719-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201792-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201792-
Descrição: dc.descriptionThere is no use restriction associated with bees for many fungicides used in agriculture; however, this does not always mean that these pesticides are harmless for these nontarget organisms. We investigated whether the fungicide pyraclostrobin, which acts on fungal mitochondria, also negatively affects honey bee mitochondrial bioenergetics. Honey bees were collected from 5 hives and anesthetized at 4 °C. The thoraces were separated, and mitochondria were isolated by grinding, filtering, and differential centrifugation. An aliquot of 0.5 mg of mitochondrial proteins was added to 0.5 mL of a standard reaction medium with 4 mM succinate (complex II substrate) plus 50 nM rotenone (complex I inhibitor), and mitochondrial respiration was measured at 30 °C using a Clark-type oxygen electrode. Mitochondrial membrane potential was determined spectrofluorimetrically using safranin O as a probe, and adenosine triphosphate (ATP) synthesis was determined by chemiluminescence. Pyraclostrobin at 0 to 50 μM was tested on the mitochondrial preparations, with 3 repetitions. Pyraclostrobin inhibited mitochondrial respiration in a dose-dependent manner at concentrations of 10 μM and above, demonstrating typical inhibition of oxidative phosphorylation. Pyraclostrobin also promoted a decline in the mitochondrial membrane potential at doses of 5 μM and above and in ATP synthesis at 15 μM and above. We conclude that pyraclostrobin interferes with honey bee mitochondrial function, which is especially critical for the energy-demanding flight activity of foraging bees. Environ Toxicol Chem 2020;39:1267–1272. © 2020 SETAC.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Animal Science College of Agricultural and Technological Sciences São Paulo State University (Unesp)-
Descrição: dc.descriptionDepartment of Genetics Ribeirão Preto Medical School University of São Paulo-
Descrição: dc.descriptionDepartment of Biosystem Engineering School of Science and Engineering São Paulo State University (Unesp)-
Descrição: dc.descriptionDepartment of Animal Science College of Agricultural and Technological Sciences São Paulo State University (Unesp)-
Descrição: dc.descriptionDepartment of Biosystem Engineering School of Science and Engineering São Paulo State University (Unesp)-
Descrição: dc.descriptionFAPESP: 2016/00446-7-
Descrição: dc.descriptionFAPESP: 2016/21818-0-
Formato: dc.format1267-1272-
Idioma: dc.languageen-
Relação: dc.relationEnvironmental Toxicology and Chemistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAdenosine triphosphate-
Palavras-chave: dc.subjectApis mellifera-
Palavras-chave: dc.subjectFungicide-
Palavras-chave: dc.subjectMitochondria-
Palavras-chave: dc.subjectStrobilurin-
Palavras-chave: dc.subjectToxicity mechanism-
Título: dc.titleMitochondrial Respiratory Inhibition Promoted by Pyraclostrobin in Fungi is Also Observed in Honey Bees-
Tipo de arquivo: dc.typelivro digital-
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