Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorRuiz, Gilia Cristine Marques [UNESP]-
Autor(es): dc.creatorPazin, Wallance Moreira [UNESP]-
Autor(es): dc.creatordo Carmo Morato, Luis Fernando [UNESP]-
Autor(es): dc.creatorOliveira, Osvaldo N.-
Autor(es): dc.creatorConstantino, Carlos José Leopoldo [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:34:27Z-
Data de disponibilização: dc.date.available2022-02-22T00:34:27Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.molliq.2020.113324-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201764-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201764-
Descrição: dc.descriptionRisks to human health have been reported owing to prolonged exposure to hormones, whose action depends on their molecular-level interaction with cell membranes. In this study, we investigate the interaction of the synthetic hormone 17 α-ethynylestradiol (EE2) in two different membrane models, Langmuir monolayers and giant unilamellar vesicles (GUVs) made with a binary mixture of 1,2-dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC) and cholesterol (Chol) in order to mimic the plasma membrane of mammalian cells. EE2 was found to expand the Langmuir monolayers, with shifts to larger areas per molecule in the surface pressure isotherm. In all of these observations, stronger effects were noted for the DPPC/Chol monolayers with XChol = 0.3, which mimics the proportion of phospholipid/sterol in the plasma membrane. At high surface pressures, EE2 is believed to weaken the attractive interactions between DPPC and Chol, in addition to affecting the ordering of the lipid chains as indicated in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) measurements. In GUVs obtained with XChol = 0.3 mixtures, EE2 induced a phase contrast loss as a result of increased permeability of the lipid bilayer. The results with Langmuir monolayers and GUV combined point to EE2 action on representative cell membranes, which can be correlated with physiological effects caused by indirect intake of EE2.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionInstituto Nacional de Ciência e Tecnologia em Eletrônica Orgânica-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Physics School of Technology and Applied Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionSao Carlos Institute of Physics University of Sao Paulo (USP)-
Descrição: dc.descriptionDepartment of Physics School of Technology and Applied Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2013/14262-7-
Descrição: dc.descriptionFAPESP: 2016/09633-4-
Descrição: dc.descriptionCAPES: 88882.330129/2018-01-
Idioma: dc.languageen-
Relação: dc.relationJournal of Molecular Liquids-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject17α-Ethynylestradiol-
Palavras-chave: dc.subjectCholesterol-
Palavras-chave: dc.subjectDPPC-
Palavras-chave: dc.subjectGiant unilamellar vesicles-
Palavras-chave: dc.subjectLangmuir monolayers-
Palavras-chave: dc.subjectMembrane model-
Palavras-chave: dc.subjectPM-IRRAS-
Título: dc.titleCorrelating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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