Morphometric analysis and pattern of protein and gene expression of apoptosis in focal cerebral ischemia in rats and the neuroprotetive action of hypothermia and ketoprofen

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUnimar-
Autor(es): dc.creatorTirapelli, Daniela P. C.-
Autor(es): dc.creatorCirino, Mucio Luiz de Assis-
Autor(es): dc.creatorCarvalho, Camila A. Melo de-
Autor(es): dc.creatorNovais, Paulo Cezar-
Autor(es): dc.creatorFigueredo, Rafael Zimak-
Autor(es): dc.creatorPorsani, Lucas Barbosa-
Autor(es): dc.creatorGula, Isabella Stracieri-
Autor(es): dc.creatorRodrigues, Andressa Romualdo-
Autor(es): dc.creatorSilva, Jairo Pinheiro da-
Autor(es): dc.creatorPereira, Adriana Lis-
Autor(es): dc.creatorNeto, Fermino Sanches Lizarte-
Autor(es): dc.creatorSchimming, Bruno Cesar [UNESP]-
Autor(es): dc.creatorTazima, Maria de Fátima Galli Sorita-
Autor(es): dc.creatorFazan, Valéria de Paula Sassoli-
Autor(es): dc.creatorCarlotti, Carlos Gilberto-
Autor(es): dc.creatorTirapelli, Luis Fernando-
Autor(es): dc.creatorColli, Benedicto Oscar-
Data de aceite: dc.date.accessioned2022-02-22T00:34:17Z-
Data de disponibilização: dc.date.available2022-02-22T00:34:17Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.4067/S0717-95022020000300523-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201707-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201707-
Descrição: dc.descriptionThis study aimed to investigate the morphometric and the pattern of protein and gene expression related to the extrinsic apoptotic pathway in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. For this analysis, 120 rats were randomly divided into 3 groups (20 animals each): control - no surgery (20 animals); sham - simulation of surgery (20 animals); ischemic - focal ischemia for 1 hour, without reperfusion (80 animals) and divided into four subgroups with 20 animals each: ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the apoptosis genes (Fas, c-Flip, caspase-8 and caspase-3) and the apoptosis protein caspase-3 were evaluated by quantitative real-time PCR and immunohistochemistry, respectively. Hypo expression of genes of extrinsic pathway of apoptosis was observed: Fas receptor, c-Flip and caspase-8 in the ischemics areas. Increases in the gene and protein caspase-3 in the ischemic areas were also observed, and these increases were reduced by hypothermia and ketoprofen, also noted in the morphometric study. The caspases-3 increase suggests that this gene plays an important role in apoptosis, probably culminating in cell death and that the neuroprotective effect of hypothermia and ketoprofen is involved.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Surgery and Anatomy FMRP-USP-
Descrição: dc.descriptionDepartment of Anatomy UNESP-
Descrição: dc.descriptionFaculty of Odontology Unimar-
Descrição: dc.descriptionDepartment of Anatomy UNESP-
Descrição: dc.descriptionFAPESP: 2005/00381-8-
Formato: dc.format523-529-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Morphology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectApoptosis-
Palavras-chave: dc.subjectCaspase-3-
Palavras-chave: dc.subjectCerebral ischemia-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectHypothermia-
Palavras-chave: dc.subjectKetoprofen-
Palavras-chave: dc.subjectNeuroprotection-
Título: dc.titleMorphometric analysis and pattern of protein and gene expression of apoptosis in focal cerebral ischemia in rats and the neuroprotetive action of hypothermia and ketoprofen-
Título: dc.titleAnálisis morfométrico y modelo de expresión de proteína y genes de apoptosis en isquemia cerebral focal en ratas y la acción neuroprotectora de hipotermia y ketoprofeno-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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