Correlating Artepillin C cytotoxic activity on HEp-2 cells with bioinspired systems of plasma membranes

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorKobal, Mirella B. [UNESP]-
Autor(es): dc.creatorPazin, Wallance M. [UNESP]-
Autor(es): dc.creatorBistaffa, Maria J. [UNESP]-
Autor(es): dc.creatorConstantino, Carlos J.L. [UNESP]-
Autor(es): dc.creatorToledo, Karina A. [UNESP]-
Autor(es): dc.creatorAoki, Pedro H.B. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:34:14Z-
Data de disponibilização: dc.date.available2022-02-22T00:34:14Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.msec.2020.110943-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201686-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201686-
Descrição: dc.descriptionArtepillin C is the main compound present in propolis from Baccharis dracunculifolia, whose antitumor activity has been the focus of many studies. Herein, we shall investigate the Artepillin C mechanisms of action against cells derived from the oropharyngeal carcinoma (HEp-2). Cytotoxicity tests revealed that the concentrations of Artepillin C required to reduce cell viability by 50% (CC50) are dependent on the incubation time, decreasing from 40.7 × 10−5 mol/L to 15.7 × 10−5 mol/L and 9.05 × 10−5 mol/L considering 12, 24 and 48 h, respectively. Hydrophobic interactions on neutral species of Artepillin C induce aggregation over the HEp-2 plasma membrane, given the acid conditions of the cellular culture. Indeed, Langmuir monolayers mimicking cellular membranes of tumor cells revealed Artepillin C affinity to interact with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) containing 20 mol% of 1,2-dipalmitoyl-sn-glychero-3-phosphoserine (DPPS), leading aggregation on giant unilamellar vesicles (GUVs) at pH 3.2. Moreover, leakage experiments on GUVs have shown that the presence of DPPS enhances the efflux of the fluorescent probe signaling the membrane permeabilization, which is the origin of the necrotic pathway triggered in HEp-2 cells, as observed by flow cytometry assays.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences Humanities and Languages-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Technology and Applied Sciences-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences Humanities and Languages-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Technology and Applied Sciences-
Descrição: dc.descriptionFAPESP: 2013/14262-7-
Descrição: dc.descriptionFAPESP: 2016/13280-0-
Descrição: dc.descriptionCNPq: 403713/2016-1-
Idioma: dc.languageen-
Relação: dc.relationMaterials Science and Engineering C-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectArtepillin C-
Palavras-chave: dc.subjectGreen propolis-
Palavras-chave: dc.subjectGUVs-
Palavras-chave: dc.subjectModel membranes-
Palavras-chave: dc.subjectOropharyngeal carcinoma cells-
Título: dc.titleCorrelating Artepillin C cytotoxic activity on HEp-2 cells with bioinspired systems of plasma membranes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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