Effects of propolis and phenolic acids on triple-negative breast cancer cell lines: Potential involvement of epigenetic mechanisms

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorAssumpção, João Henrique Maia [UNESP]-
Autor(es): dc.creatorTakeda, Agnes Alessandra Sekijima [UNESP]-
Autor(es): dc.creatorSforcin, José Maurício [UNESP]-
Autor(es): dc.creatorRainho, Cláudia Aparecida [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:34:00Z-
Data de disponibilização: dc.date.available2022-02-22T00:34:00Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-03-02-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/molecules25061289-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201618-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201618-
Descrição: dc.descriptionTriple-negative breast cancer is an aggressive disease frequently associated with resistance to chemotherapy. Evidence supports that small molecules showing DNA methyltransferase inhibitory activity (DNMTi) are important to sensitize cancer cells to cytotoxic agents, in part, by reverting the acquired epigenetic changes associated with the resistance to therapy. The present study aimed to evaluate if chemical compounds derived from propolis could act as epigenetic drugs (epi-drugs). We selected three phenolic acids (caffeic, dihydrocinnamic, and p-coumaric) commonly detected in propolis and the (-)-epigallocatechin-3-gallate (EGCG) from green tea, which is a wellknown DNA demethylating agent, for further analysis. The treatment with p-coumaric acid and EGCG significantly reduced the cell viability of four triple-negative breast cancer cell lines (BT-20, BT-549, MDA-MB-231, and MDA-MB-436). Computational predictions by molecular docking indicated that both chemicals could interact with the MTAse domain of the human DNMT1 and directly compete with its intrinsic inhibitor S-Adenosyl-L-homocysteine (SAH). Although the ethanolic extract of propolis (EEP) did not change the global DNA methylation content, by using MS-PCR (Methylation-Specific Polymerase Chain Reaction) we demonstrated that EEP and EGCG were able to partly demethylate the promoter region of RASSF1A in BT-549 cells. Also, in vitro treatment with EEP altered the RASSF1 protein expression levels. Our data indicated that some chemical compound present in the EEP has DNMTi activity and can revert the epigenetic silencing of the tumor suppressor RASSF1A. These findings suggest that propolis are a promising source for epi-drugs discovery.-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationMolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDNA methylation-
Palavras-chave: dc.subjectDNA methyltransferase inhibitors-
Palavras-chave: dc.subjectEGCG-
Palavras-chave: dc.subjectEpigenetic therapy-
Palavras-chave: dc.subjectMolecular docking-
Palavras-chave: dc.subjectRASSF1-
Título: dc.titleEffects of propolis and phenolic acids on triple-negative breast cancer cell lines: Potential involvement of epigenetic mechanisms-
Tipo de arquivo: dc.typelivro digital-
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