Antiviral activity of curcumin-nanoemulsion associated with photodynamic therapy in vulvar cell lines transducing different variants of HPV-16

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Bern-
Autor(es): dc.contributorCenter for Translational Research in Oncology- Instituto do Cancer do Estado de Sao Paulo-
Autor(es): dc.creatorBonfim, Caroline Measso do [UNESP]-
Autor(es): dc.creatorMonteleoni, Letícia Figueiredo [UNESP]-
Autor(es): dc.creatorCalmon, Marília de Freitas [UNESP]-
Autor(es): dc.creatorCândido, Natália Maria [UNESP]-
Autor(es): dc.creatorProvazzi, Paola Jocelan Scarin [UNESP]-
Autor(es): dc.creatorLino, Vanesca de Souza-
Autor(es): dc.creatorRabachini, Tatiana-
Autor(es): dc.creatorSichero, Laura-
Autor(es): dc.creatorVilla, Luisa Lina-
Autor(es): dc.creatorQuintana, Silvana Maria-
Autor(es): dc.creatorMelli, Patrícia Pereira dos Santos-
Autor(es): dc.creatorPrimo, Fernando Lucas [UNESP]-
Autor(es): dc.creatorAmantino, Camila Fernanda-
Autor(es): dc.creatorTedesco, Antonio Claudio-
Autor(es): dc.creatorBoccardo, Enrique-
Autor(es): dc.creatorRahal, Paula [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:33:51Z-
Data de disponibilização: dc.date.available2022-02-22T00:33:51Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/21691401.2020.1725023-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201554-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201554-
Descrição: dc.descriptionVulvar intraepithelial neoplasia (VIN) is associated with human papillomavirus (HPV) infection. Curcumin is a natural bioactive compound with antineoplastic properties. The use of nanoparticles containing curcumin could allow a better performance of this compound in therapies. So, VIN biopsies were collected and HPV DNA detection was performed by PCR, positive samples were genotyped by Restriction Fragment Length Polymorphism (RFLP) and HPV-16 variants were determined by sequencing. HPV-16 positive vulva carcinoma cells (A431) were transduced with E-P and E-350G HPV-16 E6 variants. The viability of the transduced cells treated with nanoemulsions was determined by MTT assay. Besides, apoptosis was evaluated by enzymatic activity of Caspase-3/7. The cell viability assay showed that both the empty nanoemulsion (NE-V) and the nanoemulsion of curcumin (NE-CUR) had little effect on cell viability as compared to control cells. Additionally, we observed that cells irradiated in the presence of NE-CUR presented 90% of cell death. The apoptosis assay further revealed a significant increase in the activity of caspases 3 and 7 in A431 cells expressing both HPV-16 E6 variants after treatment with NE-CUR. Finally, we submitted the HPV transduced A431 cells to organotypic cultures and observed that the combination of treatments affected tissue architecture with evident signals of tissue damage. We concluded that nanoemulsions attain good biocompatibility, since no cytotoxicity was observed and NE-CUR associated with photoactivation showed promising results, leading to death only in cells subjected to irradiation. This drug delivery system associated with photodynamic therapy may become promising in the treatment of vulva lesions.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionLaboratory of Genomic Studies Sao Paulo State University–UNESP-
Descrição: dc.descriptionDepartment of Microbiology Institute of Biomedical Sciences University of São Paulo–USP-
Descrição: dc.descriptionInstitute of Pharmacology University of Bern-
Descrição: dc.descriptionICESP Center for Translational Research in Oncology- Instituto do Cancer do Estado de Sao Paulo-
Descrição: dc.descriptionDepartament of Radiology and Oncology School of Medicine Universidade de Sao Paulo–USP-
Descrição: dc.descriptionDepartment of Gynecology and Obstetrics of the Ribeirão Preto Medical School USP Ribeirão Preto-
Descrição: dc.descriptionDepartment of Bioprocess and Biotechnology Faculty of Pharmaceutical Sciences of Araraquara Universidade Estadual Paulista Julio de Mesquita Filho–UNESP Araraquara-
Descrição: dc.descriptionDepartment of Chemistry Center for Nanotechnology and Tissue Engineering Faculty of Philosophy Sciences and Languages ​​of Ribeirão Preto University of São Paulo–USP Ribeirão Preto-
Descrição: dc.descriptionLaboratory of Genomic Studies Sao Paulo State University–UNESP-
Descrição: dc.descriptionDepartment of Bioprocess and Biotechnology Faculty of Pharmaceutical Sciences of Araraquara Universidade Estadual Paulista Julio de Mesquita Filho–UNESP Araraquara-
Descrição: dc.descriptionFAPESP: 2014/02064-9-
Formato: dc.format515-524-
Idioma: dc.languageen-
Relação: dc.relationArtificial Cells, Nanomedicine and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcurcumin and photodynamic therapy-
Palavras-chave: dc.subjectHPV-
Palavras-chave: dc.subjectnanoemulsion-
Palavras-chave: dc.subjectVulvar intraepithelial neoplasia-
Título: dc.titleAntiviral activity of curcumin-nanoemulsion associated with photodynamic therapy in vulvar cell lines transducing different variants of HPV-16-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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