Ovum pick-up and in vitro maturation in spotted paca (Cuniculus paca—Linnaeus, 1766)

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorRio de Janeiro Rural Federal University - UFRRJ-
Autor(es): dc.contributorUniversidade Federal do Pará (UFPA)-
Autor(es): dc.creatorBarros, Felipe Farias Pereira da Câmara [UNESP]-
Autor(es): dc.creatorTeixeira, Pedro Paulo Maia-
Autor(es): dc.creatorPadilha-Nakaghi, Luciana Cristina [UNESP]-
Autor(es): dc.creatorUscategui, Ricardo Andres Ramirez [UNESP]-
Autor(es): dc.creatorLima, Marina Ragagnin [UNESP]-
Autor(es): dc.creatorSantos, Victor José Correia [UNESP]-
Autor(es): dc.creatorRossy, Kayan da Cunha-
Autor(es): dc.creatorBorges, Luísa Pucci Bueno-
Autor(es): dc.creatorMachado, Marcia Rita Fernandes [UNESP]-
Autor(es): dc.creatorVicente, Wilter Ricardo Russiano [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:33:44Z-
Data de disponibilização: dc.date.available2022-02-22T00:33:44Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/rda.13627-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201512-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201512-
Descrição: dc.descriptionWe tested FSHp, eCG and FSHp + eCG to establish ovum pick-up (OPU) and in vitro maturation method in spotted paca. Eight healthy adult females were subjected to each of four treatments to stimulate ovarian follicular growth. All females were subjected to a hormonal protocol using a single dose of 45 mg of injectable progesterone and single intramuscular injection of 0.075 mg d-cloprostenol on day 6. Ovarian stimulation was carried out as follows: in Group TFE (FSHp and eCG), animals were treated with a single dose of 80 mg of FSHp and 200 IU of eCG intramuscularly on day 6 after the application of progesterone; in Group TF (FSHp), they were treated with a single dose of 80 mg of FSHp intramuscularly on day 6 after application of progesterone; in Group treatment eCG, they were treated with 200 IU of eCG intramuscularly on day 6 after application of progesterone; and in Group TC (saline solution), 1 ml of saline solution was administered to control does. The OPU was performed between 22 and 26 hr after gonadotropin treatments. All recovered oocytes were placed into maturation media and incubated for 24 hr. There were no differences among the mean number of observed follicles, aspirated follicles and oocytes recovered per treatment. Oocyte maturation rates did not differ among groups, except, TF and treatment eCG oocytes had greater maturation rates than TC oocytes. In this study, gonadotropin administration failed to superovulate treated does and increase oocyte retrieval efficiency. Despite the feasibility of the procedure, further studies are needed to develop and refine hormonal protocols for oocyte recovery and in vitro maturation in this species.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCollege of Agricultural and Veterinary Science (FCAV) UNESP Univ Estadual Paulista-
Descrição: dc.descriptionMedicine and Veterinary Surgery Department Rio de Janeiro Rural Federal University - UFRRJ-
Descrição: dc.descriptionVeterinary Institute Pará Federal University - UFPA-
Descrição: dc.descriptionCollege of Agricultural and Veterinary Science (FCAV) UNESP Univ Estadual Paulista-
Formato: dc.format442-447-
Idioma: dc.languageen-
Relação: dc.relationReproduction in Domestic Animals-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectfollicular aspiration-
Palavras-chave: dc.subjectgonadotropin-
Palavras-chave: dc.subjecthormonal protocol-
Palavras-chave: dc.subjectoocyte maturation-
Palavras-chave: dc.subjectrodent-
Título: dc.titleOvum pick-up and in vitro maturation in spotted paca (Cuniculus paca—Linnaeus, 1766)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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