β-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorFrassatto, Priscila Aparecida Casciatori [UNESP]-
Autor(es): dc.creatorCasciatori, Fernanda Perpétua-
Autor(es): dc.creatorThoméo, João Cláudio [UNESP]-
Autor(es): dc.creatorGomes, Eleni [UNESP]-
Autor(es): dc.creatorBoscolo, Maurício [UNESP]-
Autor(es): dc.creatorda Silva, Roberto [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:33:41Z-
Data de disponibilização: dc.date.available2022-02-22T00:33:41Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s13399-020-00608-1-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201491-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201491-
Descrição: dc.descriptionFor degradation of sugarcane bagasse (SCB), several enzymes are needed but β-glucosidase is rate limiting in cellulose hydrolysis. Since different microorganisms synthetize characteristic pool of enzymes, mixing extracts produced by different species may increase hydrolytic efficiency due to synergism between enzymes in cocktails. This paper reports the study of β-glucosidase production in solid state cultivation (SSC) of two filamentous fungi, thermophilic Thermoascus aurantiacus and mesophilic Trichoderma reesei, and application of the enzymatic extracts on non-pretreated SCB saccharification. Enzyme extract obtained from the thermophilic fungus presented higher β-glucosidase and FPU activities (1.8 U/mL and 10 FPU/mL) than the one from mesophilic (0.2 U/mL and 6 FPU/mL). Optimal SCB hydrolysis was achieved when applying enzymatic cocktail composed of equal volumes of both fungal extracts (3.6 FPU/gSCB, filter paper units per gram SCB, 2.25 FPU/gSCB provided by extract from T. aurantiacus and 1.35 FPU/gSCB from T. reesei) at 65 °C. The hydrolysis yield applying the enzyme cocktail, 124 mg total reducing sugars (TRS) per gSCB, was higher than any yield achieved when using the enzyme extracts separately (105 mgTRS/gSCB using 12.5 FPU per gSCB from T. aurantiacus at 65 °C; 79 mgTRS/gSCB using 7.5 FPU per gSCB from T. reesei at 45 °C). Therefore, the use of the cocktail (3.6 FPU/gSCB) at 65 °C released 18 and 57% more TRS respectively than when extracts from T. aurantiacus or from T. reesei were applied alone, respectively, even reducing enzyme load (FPU) by 70%, corroborating the synergistic effect when both extracts are used together.-
Descrição: dc.descriptionDepartment of Chemistry and Environmental Sciences Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo 2265-
Descrição: dc.descriptionDepartment of Chemical Engineering Federal University of São Carlos (UFSCar), Rodovia Washington Luís km 235 (SP 310)-
Descrição: dc.descriptionDepartment of Food Engineering and Technology Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo 2265-
Descrição: dc.descriptionDepartment of Biology Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo 2265-
Descrição: dc.descriptionDepartment of Chemistry and Environmental Sciences Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo 2265-
Descrição: dc.descriptionDepartment of Food Engineering and Technology Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo 2265-
Descrição: dc.descriptionDepartment of Biology Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo 2265-
Idioma: dc.languageen-
Relação: dc.relationBiomass Conversion and Biorefinery-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiofuels-
Palavras-chave: dc.subjectEnzymes-
Palavras-chave: dc.subjectFermentation biotechnology-
Palavras-chave: dc.subjectFungal growth-
Palavras-chave: dc.subjectHydrolysis-
Título: dc.titleβ-Glucosidase production by Trichoderma reesei and Thermoascus aurantiacus by solid state cultivation and application of enzymatic cocktail for saccharification of sugarcane bagasse-
Tipo de arquivo: dc.typelivro digital-
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