Triiodothyronine activated extranuclear pathways upregulate adiponectin and leptin in murine adipocytes

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorMathias, Lucas Solla [UNESP]-
Autor(es): dc.creatorRodrigues, Bruna Moretto [UNESP]-
Autor(es): dc.creatorGonçalves, Bianca Mariani [UNESP]-
Autor(es): dc.creatorMoretto, Fernanda Cristina Fontes [UNESP]-
Autor(es): dc.creatorOlimpio, Regiane Marques Castro [UNESP]-
Autor(es): dc.creatorDeprá, Igor [UNESP]-
Autor(es): dc.creatorDe Sibio, Maria Teresa [UNESP]-
Autor(es): dc.creatorTilli, Helena Paim [UNESP]-
Autor(es): dc.creatorNogueira, Célia Regina [UNESP]-
Autor(es): dc.creatorde Oliveira, Miriane [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:33:30Z-
Data de disponibilização: dc.date.available2022-02-22T00:33:30Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.mce.2019.110690-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201427-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201427-
Descrição: dc.descriptionAdiponectin and leptin, important for metabolic regulation, are synthesized and secreted by adipose tissue and are influenced by triiodothyronine (T3) that activates the MAPK/ERK and integrin αVβ3 pathways, modulating gene expression. Adipocytes were treated with T3 (10 nM), for 1 h, in the absence or presence of PD98059 (PD) and tetraiodothyroacetic acid (Tetrac), which are pathways inhibitors. The cells were incubated with Adipo Red/Oil Red O reagents, and intracellular lipid accumulation [glycerol and triacylglycerol (TAG)], MTT, 8-hydroxideoxyguanosine (8-OH-dG), and mRNA and protein expression were assessed. T3 increased leptin mRNA and protein expression, and, in contrast, there was a decrease in the Tetrac + T3 group. Adiponectin mRNA expression was not altered by T3, though it had increased its protein expression, which was terminated by inhibitors PD + T3 and Tetrac + T3. However, T3 did not alter PPARγ protein expression, lipid accumulation, TAG, glycerol, and DNA damage, but PD + T3 and Tetrac + T3 reduced these parameters. T3 activated the MAPK/ERK pathway on adipocytes to modulate the adiponectin protein expression and integrin αvβ3 to alter the leptin gene expression.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (UNESP) Botucatu Medical School-
Descrição: dc.descriptionSão Paulo State University (UNESP) Botucatu Medical School-
Descrição: dc.descriptionFAPESP: 2010/16911-4-
Idioma: dc.languageen-
Relação: dc.relationMolecular and Cellular Endocrinology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAdiponectin-
Palavras-chave: dc.subjectIntegrin αVβ3-
Palavras-chave: dc.subjectLeptin-
Palavras-chave: dc.subjectLipid-
Palavras-chave: dc.subjectMAPK/ERK-
Palavras-chave: dc.subjectTriiodothyronine-
Título: dc.titleTriiodothyronine activated extranuclear pathways upregulate adiponectin and leptin in murine adipocytes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.