Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorMelo, Larissa Martins [UNESP]-
Autor(es): dc.creatorBragato, Jaqueline Poleto [UNESP]-
Autor(es): dc.creatorVenturin, Gabriela Lovizutto [UNESP]-
Autor(es): dc.creatorRebech, Gabriela Torres [UNESP]-
Autor(es): dc.creatorCosta, Sidnei Ferro [UNESP]-
Autor(es): dc.creatorGarcia, Leandro Encarnacão [UNESP]-
Autor(es): dc.creatorLopes, Flávia Lombardi [UNESP]-
Autor(es): dc.creatorDe Rezende Eugênio, Flavia [UNESP]-
Autor(es): dc.creatorSantos, Paulo Sergio Patto Dos [UNESP]-
Autor(es): dc.creatorDe Lima, Valéria Marçal Felix [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:33:25Z-
Data de disponibilização: dc.date.available2022-02-22T00:33:25Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0226192-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201391-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201391-
Descrição: dc.descriptionVisceral Leishmaniasis is a chronic zoonosis and, if left untreated, can be fatal. Infected dogs have decreased cellular immunity (Th1) and develop a potent humoral response (Th2), which is not effective for elimination of the protozoan. Immune response can be modulated by microRNAs (miRNAs), however, characterization of miRNAs and their possible regulatory role in the spleen of infected dogs have not been done. We evaluated miRNA expression in splenic leukocytes (SL) from dogs naturally infected with Leishmania infantum and developing leishmaniasis (CanL; n = 8) compared to healthy dogs (n = 4). Microarray analysis showed increased expression of miR 21, miR 148a, miR 7 and miR 615, and downregulation of miR 150, miR 125a and miR 125b. Real-time PCR validated the differential expression of miR 21, miR 148a and miR 615. Further, decrease of miR 21 in SL, by means of transfection with a miR 21 inhibitor, increased the IL-12 cytokine and the T-bet/GATA-3 ratio, and decreased parasite load on SL of dogs with CanL. Taken together, these findings suggest that L. infantum infection alters splenic expression of miRNAs and that miR 21 interferes in the cellular immune response of L. infantum-infected dogs, placing this miRNA as a possible therapeutic target in CanL.-
Descrição: dc.descriptionDepartment of Animal Clinic Surgery and Reproduction São Paulo State University (Unesp) School of Veterinary Medicine-
Descrição: dc.descriptionDepartment of Production and Animal Health São Paulo State University (Unesp) School of Veterinary Medicine-
Descrição: dc.descriptionDepartment of Animal Clinic Surgery and Reproduction São Paulo State University (Unesp) School of Veterinary Medicine-
Descrição: dc.descriptionDepartment of Production and Animal Health São Paulo State University (Unesp) School of Veterinary Medicine-
Idioma: dc.languageen-
Relação: dc.relationPLoS ONE-
???dc.source???: dc.sourceScopus-
Título: dc.titleInduction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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