Biotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Havana-
Autor(es): dc.contributorAIDS Research Laboratory (LISIDA)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorTamayo, Abel I. Balbin-
Autor(es): dc.creatorRizo, Laura S. López-
Autor(es): dc.creatorde Armas, Madelin Blanco-
Autor(es): dc.creatorFerreira, Antonio Aparecido Pupim [UNESP]-
Autor(es): dc.creatorManzani, Danilo-
Autor(es): dc.creatorYamanaka, Hideko [UNESP]-
Autor(es): dc.creatorGuas, Ana Margarita Esteva-
Data de aceite: dc.date.accessioned2022-02-22T00:33:22Z-
Data de disponibilização: dc.date.available2022-02-22T00:33:22Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.microc.2019.104462-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201371-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201371-
Descrição: dc.descriptionThis study was performed to prove the feasibility of a direct impedimetric DNA genosensor by using biotin self-assembled monolayer (SAM). The sensor was composed of a gold rod with a SAM of biotin-modified 19-base oligonucleotide probe (5´-AAA GAT TAA TCC TGG G -3´,) for HIV-1. The electrode modification was characterized by Surface-Enhancement Raman Spectroscopy (SERS), cyclic voltammetry and the hybridization with the complementary oligonucleotide was monitored by impedimetric technique. SERS was used to confirm the linking of Biotin-DNA on the surface of the Au electrode. The coverage was 89% of the surface and a concentration of complementary oligonucleotides of 2.5 10− 12 molL−1 could be detected.-
Descrição: dc.descriptionDepartment of Analytical Chemistry University of Havana-
Descrição: dc.descriptionAIDS Research Laboratory (LISIDA)-
Descrição: dc.descriptionDepartment of Analytical Chemistry São Paulo State University (UNESP)-
Descrição: dc.descriptionSão Carlos Institute of Chemistry Department of Chemistry and Molecular Physics University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Analytical Chemistry São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationMicrochemical Journal-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDNA biosensors-
Palavras-chave: dc.subjectGenosensor-
Palavras-chave: dc.subjectHIV-1-
Palavras-chave: dc.subjectHybridization-
Palavras-chave: dc.subjectImpedimetric-
Palavras-chave: dc.subjectProviral DNA-
Título: dc.titleBiotin self-assembled monolayer for impedimetric genosensor for direct detection of HIV-1-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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