Uncommon Ehrlichia canis infection associated with morulae in neutrophils from naturally infected dogs in Brazil

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Mato Grosso (UFMT)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Cuiabá (UNIC)-
Autor(es): dc.contributorUniversidade Federal do Paraná (UFPR)-
Autor(es): dc.creatorAguiar, Daniel Moura-
Autor(es): dc.creatorRodrigues, Fernanda Panseri [UNESP]-
Autor(es): dc.creatorRibeiro, Márcio Garcia [UNESP]-
Autor(es): dc.creatordos Santos, Bruna [UNESP]-
Autor(es): dc.creatorMuraro, Livia Saab-
Autor(es): dc.creatorTaques, Isis Indaiara Gonçalves Granjeiro-
Autor(es): dc.creatorCampos, Amanda Noeli Silva-
Autor(es): dc.creatorDutra, Valéria-
Autor(es): dc.creatorNakazato, Luciano-
Autor(es): dc.creatorda Costa Vieira, Rafael Felipe-
Autor(es): dc.creatorTakahira, Regina Kiomi [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:33:14Z-
Data de disponibilização: dc.date.available2022-02-22T00:33:14Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/tbed.13390-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201330-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201330-
Descrição: dc.descriptionEhrlichia and Anaplasma species are the most common tick-borne disease (TBD) pathogens in dogs worldwide. Ehrlichia canis, the aetiological agent of the Canine Monocytic Ehrlichiosis (CME), is known to replicate within the cytoplasm of mononuclear cells into clusters of organisms called morulae. However, detection of morulae in neutrophils is commonly observed in dogs infected by Ehrlichia ewingii or Anaplasma phagocytophilum. We report uncommon clinical cases of canine ehrlichiosis presenting morulae compatible with E. ewingii and A. phagocytophilum in dogs from two distinct regions of Brazil. Eight dogs were admitted to two veterinary teaching hospitals from Brazil, showing clinical or haematological signs suggestive of TBD. Blood or peritoneal fluid was withdrawn for haematological and cytologic analysis. All samples were evaluated by PCR assays for Ehrlichia and Anaplasma using genus-specific primers for dsb, 16S rRNA and groEL genes, followed by sequencing. Samples were also evaluated by nested PCR assays for the 16S rRNA gene of E. ewingii and groEL gene of A. phagocytophilum and Anaplasma platys. Seven dogs revealed thrombocytopenia, six dogs had monocytosis and five presented lymphopenia and anaemia. All dogs showed morulae structures compatible with Ehrlichia spp. in neutrophils and were PCR-positive for the dsb and 16S rRNA gene fragments of Ehrlichia, with sequences showing 100% identity with multiple E. canis sequences deposited in the GenBank™. Sequencing of 16S rRNA and groEL gene fragments from one PCR-positive dog showed 100% identity with A. platys. Overall, our data suggest that in endemic regions for E. canis, that is Brazil, the presence of morulae in neutrophils may indicate infection by this bacterium. Herein, morulae were also found in neutrophils present in the peritoneal fluid of a dog. Also, this is the first report of E. canis and Hepatozoon canis co-infection in neutrophils from naturally infected dogs confirmed by DNA sequencing.-
Descrição: dc.descriptionLaboratory of Virology and Rickettsial Infections Veterinary Hospital Federal University of Mato Grosso (UFMT)-
Descrição: dc.descriptionVeterinary Clinical Laboratory Department of Veterinary Clinics São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Veterinary Hygiene and Public Health São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory of Clinical Pathology Veterinary Medicine College University of Cuiabá (UNIC)-
Descrição: dc.descriptionLaboratory of Microbiology and Molecular Biology Veterinary Hospital of the Faculty of Veterinary Medicine Federal University of Mato Grosso (UFMT)-
Descrição: dc.descriptionDepartment of Veterinary Medicine Federal University of Paraná (UFPR)-
Descrição: dc.descriptionVeterinary Clinical Laboratory Department of Veterinary Clinics São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Veterinary Hygiene and Public Health São Paulo State University (UNESP)-
Formato: dc.format135-141-
Idioma: dc.languageen-
Relação: dc.relationTransboundary and Emerging Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnaplasma phagocytophilum-
Palavras-chave: dc.subjectAnaplasma platys-
Palavras-chave: dc.subjectcanine ehrlichiosis-
Palavras-chave: dc.subjectEhrlichia ewingii-
Palavras-chave: dc.subjectHepatozoon canis-
Título: dc.titleUncommon Ehrlichia canis infection associated with morulae in neutrophils from naturally infected dogs in Brazil-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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