Development of a real-time PCR assay for detection of Cryptosporidium canis in dog fecal samples

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorHomem, Camila Guariz [UNESP]-
Autor(es): dc.creatordo Nascimento, Isabela Garcia [UNESP]-
Autor(es): dc.creatorSantana, Bruna Nicoleti [UNESP]-
Autor(es): dc.creatorMeireles, Marcelo Vasconcelos [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:33:08Z-
Data de disponibilização: dc.date.available2022-02-22T00:33:08Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.vprsr.2019.100345-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201290-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201290-
Descrição: dc.descriptionCryptosporidiosis is an emerging zoonotic disease caused by the worldwide distributed parasitic protozoa Cryptosporidium spp. The host-adapted species Cryptosporidium canis is most frequently found in dogs, although human infections with this species have been described. This study aimed to develop a real-time PCR targeting the HSP70 protein gene for C. canis DNA detection in dog fecal samples collected from two municipalities in the state of São Paulo, Brazil. Furthermore, the occurrence of Cryptosporidium spp. and. C. canis was also determined by nested PCR. Fecal samples from 367 dogs (21 puppies and 346 adults) were purified by water-ether sedimentation. A real-time PCR protocol targeting the HSP70 gene for the species-specific detection of C. canis was developed and compared with nested PCR results. Real-time PCR identified C. canis in 15.3% (58/367) samples. Nested PCR revealed that 10.4% (38/367) of samples were positive for Cryptosporidium spp. All sequenced 18S rRNA amplicons were C. canis. There was a higher prevalence of Cryptosporidium spp. and C. canis in puppies compared to adult dogs. No non-specific amplification was observed in C. canis specific real-time PCR assay.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) Faculdade de Medicina Veterinária, Rua Clóvis Pestana, n° 793, CEP 16050-680, Bairro Dona Amélia-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) Faculdade de Medicina Veterinária, Rua Clóvis Pestana, n° 793, CEP 16050-680, Bairro Dona Amélia-
Descrição: dc.descriptionFAPESP: 2011/08814-1-
Idioma: dc.languageen-
Relação: dc.relationVeterinary Parasitology: Regional Studies and Reports-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCryptosporidium canis-
Palavras-chave: dc.subjectDiagnosis-
Palavras-chave: dc.subjectDogs-
Palavras-chave: dc.subjectHSP70 gene-
Palavras-chave: dc.subjectReal-time PCR-
Título: dc.titleDevelopment of a real-time PCR assay for detection of Cryptosporidium canis in dog fecal samples-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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