Tuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pH

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversité de Montréal-
Autor(es): dc.creatorSemensato, Juliana [UNESP]-
Autor(es): dc.creatorFernandes, Júlio Cesar-
Autor(es): dc.creatorBenderdour, Mohamed-
Autor(es): dc.creatorde Oliveira Tiera, Vera Aparecida [UNESP]-
Autor(es): dc.creatorLima, Aline Margarete Furuyama [UNESP]-
Autor(es): dc.creatorTiera, Marcio José [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:33:06Z-
Data de disponibilização: dc.date.available2022-02-22T00:33:06Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s13233-020-8019-y-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201276-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201276-
Descrição: dc.descriptionThe improvement of biological properties of polycations is a fundamental step to overcome their limitations as non-viral gene carriers. This work studied the effect of phosphorylcholine (PC) groups on the physicochemical properties of poly(L-lysine) (PLL)/pDNA nanoparticles. Phosphorylcholine-grafted PLL derivatives (PLL-PC) containing increasing proportions of PC were obtained by the reductive amination reaction with phosphoryl glyceraldehyde and characterized by 1H NMR, FTIR, and GPC measurements. The PLL-PC derivatives were used to prepare polyplexes with pDNA and their properties were evaluated by fluorescence, gel electrophoresis and dynamic light scattering (DLS) measurements. The PLL-PC derivatives were able to interact with pDNA at low N/P ratios in physiological pH to form stable polyplexes having lower zeta potentials, as evidenced by the gel electrophoresis and zeta potentials measurements. A degree of grafting of 10% increased the in vitro transfection efficiency of PLL and a degree of 20 mol% of PC groups provided colloidal stability in physiological saline solution at neutral pH. Overall, the PC-PLL derivatives exhibited improved physicochemical properties and have significant potential for further studies as non-viral gene transfer agents. [Figure not available: see fulltext.].-
Descrição: dc.descriptionDepartamento de Química e Ciências Ambientáis UNESP-Universidade Estadual Paulista-
Descrição: dc.descriptionOrthopedic Research Laboratory Hôpital du Sacré-Coeur de Montréal Université de Montréal-
Descrição: dc.descriptionDepartamento de Química e Ciências Ambientáis UNESP-Universidade Estadual Paulista-
Formato: dc.format126-135-
Idioma: dc.languageen-
Relação: dc.relationMacromolecular Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectnanoparticles-
Palavras-chave: dc.subjectnon-viral gene therapy-
Palavras-chave: dc.subjectphosphorylcholine-
Palavras-chave: dc.subjectPLL-
Título: dc.titleTuning with Phosphorylcholine Grafts Improves the Physicochemical Properties of PLL/pDNA Nanoparticles at Neutral pH-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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