Influence of cAMP modulator supplementation of in vitro culture medium on Bos taurus indicus embryos

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorLaboratory of Animal Reproduction-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity ABC Federal-
Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorPurdue University-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal do ABC (UFABC)-
Autor(es): dc.creatorCosta, Camila Bortoliero-
Autor(es): dc.creatorLunardelli, Paula Alvares-
Autor(es): dc.creatorFontes, Patricia Kubo [UNESP]-
Autor(es): dc.creatorSudano, Mateus José-
Autor(es): dc.creatorGouveia Nogueira, Marcelo Fábio [UNESP]-
Autor(es): dc.creatorAlfieri, Amauri Alcindo-
Autor(es): dc.creatorFerreira, Christina Ramires-
Autor(es): dc.creatorde Lima, Camila Bruna-
Autor(es): dc.creatorMarinho, Luciana Simões Rafagnin-
Autor(es): dc.creatorSeneda, Marcelo Marcondes-
Data de aceite: dc.date.accessioned2022-02-22T00:32:49Z-
Data de disponibilização: dc.date.available2022-02-22T00:32:49Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2019.09.007-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201178-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201178-
Descrição: dc.descriptionThe effectiveness of the use of natriuretic peptide C (NPPC) in the blocking of meiosis has already been proven in several species. However, there are no reports on the use of NPPC in the activation of metabolic processes in embryos. Whereas modulations of cAMP concentrations alter the lipid metabolism of bovine oocytes, the present study aims to evaluate the effect of NPPC on the development, lipid content and transcript levels of genes related to lipid metabolism of IVP bovine embryos. For this purpose, ovaries were obtained from a slaughterhouse, and oocytes were fertilized in vitro (D0). From D5 of in vitro culture, embryos were treated with 100 nM NPPC (NPPC group) or with no NPPC (Control group) and evaluated in terms of Blastocyst (D7) and hatching rates (D10). For the assessment of the cytoplasmatic lipid amounts, blastocysts were stained with Sudan Black B dye. The embryonic lipid profile was investigated by electrospray ionization desorption-mass spectrometry (DESI-MS). The abundance of nine transcripts related to lipid metabolism were assessed using the Biomark HD system. For statistical analysis, blastocyst and hatching rates, lipid content by the Sudan Black B and variation of gene expression between groups were compared by Student t-test. For lipid profile analysis, principal component analysis (PCA) and fold-change were performed. The embryo lipid content was similar between NPPC (881 ± 3.7) and Control (883 ± 5.2) groups (p > 0.05). However, cholesteryl esters and TAGs were downregulated by NPPC at multiple levels according to the DESI-MS profiles. Of the analyzed genes, ELOVL6 and SREBF1 showed an up-regulation in the control group (p < 0.05), while CPT2 was observed to be up-regulated in the NPPC-treated embryos. There was no significant difference in the blastocyst production rate between NPPC (44.4%) and Control (42.4%), however the hatching rate at D10 was higher (p < 0.05) in the NPPC group (69.77%) when compared to the Control group (48.33%). These findings demonstrate that NPPC alters the mRNA expression of genes related to lipid metabolism and that it exerts a positive effect on the hatching rates of IVP Bos taurus indicus embryos.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniversity of Londrina (UEL) Laboratory of Animal Reproduction-
Descrição: dc.descriptionSão Paulo State University (Unesp) Institute of Biosciences Department of Pharmacology-
Descrição: dc.descriptionCenter for Natural and Human Sciences University ABC Federal-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences Humanities and Languages Department of Biological Science-
Descrição: dc.descriptionLaboratório de Virologia Animal Departamento de Medicina Veterinária Preventiva Universidade Estadual de Londrina-
Descrição: dc.descriptionDepartment of Chemistry and Center for Analytical Instrumentation Development Purdue University-
Descrição: dc.descriptionInstituto de Ciências Biomédicas Universidade de São Paulo-
Descrição: dc.descriptionCentro de Ciências Naturais e Humanas Universidade Federal Do ABC-
Descrição: dc.descriptionSão Paulo State University (Unesp) Institute of Biosciences Department of Pharmacology-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences Humanities and Languages Department of Biological Science-
Descrição: dc.descriptionCNPq: 403862/2016-7-
Formato: dc.format134-141-
Idioma: dc.languageen-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBovine-
Palavras-chave: dc.subjectDesorption electrospray ionization-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectIVEP-
Palavras-chave: dc.subjectLipid content-
Palavras-chave: dc.subjectNPPC-
Título: dc.titleInfluence of cAMP modulator supplementation of in vitro culture medium on Bos taurus indicus embryos-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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