Topical buparvaquone nano-enabled hydrogels for cutaneous leishmaniasis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Portsmouth-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidad CEU Cardenal Herrera-
Autor(es): dc.contributorUniversidad Complutense de Madrid-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity Complutense de Madrid-
Autor(es): dc.creatorLalatsa, Aikaterini-
Autor(es): dc.creatorStatts, Larry-
Autor(es): dc.creatorAdriana de Jesus, Jéssica-
Autor(es): dc.creatorAdewusi, Olivia-
Autor(es): dc.creatorAuxiliadora Dea-Ayuela, Maria-
Autor(es): dc.creatorBolas-Fernandez, Francisco-
Autor(es): dc.creatorDalastra Laurenti, Marcia-
Autor(es): dc.creatorFelipe Domingues Passero, Luiz [UNESP]-
Autor(es): dc.creatorSerrano, Dolores R. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:32:45Z-
Data de disponibilização: dc.date.available2022-02-22T00:32:45Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-10-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ijpharm.2020.119734-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201153-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201153-
Descrição: dc.descriptionLeishmaniasis is a neglected disease presenting cutaneous, mucosal and visceral forms and affecting an estimated 12 million mostly low-income people. Treatment of cutaneous leishmaniasis (CL) is recommended to expedite healing, reduce risk of scarring, prevent parasite dissemination to other mucocutaneous (common with New World species) or visceral forms and reduce the chance of relapse, but remains an unmet need. Available treatments are painful, prolonged (>20 days) and require hospitalisation, which increases the cost of therapy. Here we present the development of optimised topical self-nanoemulsifying drug delivery systems (SNEDDS) loaded with buparvaquone (BPQ, a hydroxynapthoquinone from the open Malaria Box) for the treatment of CL from New World species. The administration of topical BPQ-SNEDDS gels for 7 days resulted in a reduction of parasite load of 99.989 ± 0.019% similar to the decrease achieved with intralesionally administered Glucantime® (99.873 ± 0.204%) in a L. amazonensis BALB/c model. In vivo efficacy was supported by ex vivo permeability and in vivo tape stripping studies. BPQ-SNEDDS and their hydrogels demonstrated linear flux across non-infected CD-1 mouse skin ex vivo of 182.4 ± 63.0 μg cm−2 h−1 and 57.6 ± 10.8 μg cm−2 h−1 respectively localising BPQ within the skin in clinically effective concentrations (227.0 ± 45.9 μg and 103.8 ± 33.8 μg) respectively. These levels are therapeutic as BPQ-SNEDDS and their gels showed nanomolar in vitro efficacy against L. amazonensis and L. braziliensis amastigotes with excellent selectivity index toward parasites versus murine macrophages. In vivo tape stripping experiments indicated localisation of BPQ within the stratum corneum and dermis. Histology studies confirmed the reduction of parasitism and indicated healing in animals treated with BPQ-SNEDDS hydrogels. These results highlight the potential clinical capability of nano-enabled BPQ hydrogels towards a non-invasive treatment for CL.-
Descrição: dc.descriptionBiomaterials Bio-engineering and Nanomedicines (BioN) Laboratory Institute of Biomedical and Biomolecular Sciences School of Pharmacy and Biomedical Sciences University of Portsmouth, White Swan Road-
Descrição: dc.descriptionLaboratory of Pathology of Infectious Diseases (LIM-50) Medical School University of São Paulo, Avenida Dr. Arnaldo 455, 01246903 Cerqueira César-
Descrição: dc.descriptionDepartamento de Farmacia Facultad de Ciencias de la Salud Universidad CEU Cardenal Herrera, Edificio Seminario s/n, 46113-Moncada-
Descrição: dc.descriptionDepartament of Microbiology and Parasitology School of Pharmacy Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n-
Descrição: dc.descriptionInstitute of Biosciences São Paulo State University (UNESP) Praça Infante Dom Henrique s/n-
Descrição: dc.descriptionInstitute for Advanced Studies of Ocean Biosciences São Paulo State University (UNESP), Av. João Francisco Bensdorp, 1178-
Descrição: dc.descriptionDepartament of Pharmaceutics and Food Technology and Instituto Universitario de Farmacia Industrial (IUFI) School of Pharmacy University Complutense de Madrid, Plaza Ramón y Cajal s/n-
Descrição: dc.descriptionInstitute of Biosciences São Paulo State University (UNESP) Praça Infante Dom Henrique s/n-
Descrição: dc.descriptionInstitute for Advanced Studies of Ocean Biosciences São Paulo State University (UNESP), Av. João Francisco Bensdorp, 1178-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Pharmaceutics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBuparvaquone-
Palavras-chave: dc.subjectCutaneous Leishmaniasis-
Palavras-chave: dc.subjectFranz cell diffusion assays-
Palavras-chave: dc.subjectHydrogels-
Palavras-chave: dc.subjectMucocutaneous Leishmaniasis-
Palavras-chave: dc.subjectSelf-nanoemulsifying drug delivery systems (SNEDDS)-
Palavras-chave: dc.subjectTape stripping-
Título: dc.titleTopical buparvaquone nano-enabled hydrogels for cutaneous leishmaniasis-
Tipo de arquivo: dc.typelivro digital-
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