Antibiofilm and anti-caries effects of an experimental mouth rinse containing Matricaria chamomilla L. extract under microcosm biofilm on enamel

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorBraga, Aline Silva-
Autor(es): dc.creatorSimas, Leticia Lobo de Melo-
Autor(es): dc.creatorPires, Juliana Gonçalves-
Autor(es): dc.creatorSouza, Beatriz Martines-
Autor(es): dc.creatorde Melo, Fernanda Pereira de Souza Rosa [UNESP]-
Autor(es): dc.creatorSaldanha, Luiz Leonardo [UNESP]-
Autor(es): dc.creatorDokkedal, Anne Ligia [UNESP]-
Autor(es): dc.creatorMagalhães, Ana Carolina-
Data de aceite: dc.date.accessioned2022-02-22T00:32:43Z-
Data de disponibilização: dc.date.available2022-02-22T00:32:43Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2020.103415-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201141-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201141-
Descrição: dc.descriptionObjective: This study evaluated the antibiofilm and anti-caries effects of an experimental mouth rinse containing aqueous extract of Matricaria chamomilla L. Methods: Microcosm biofilm was produced on bovine enamel, from pooled human saliva mixed with McBain saliva, under 0.2 % sucrose exposure, for 5 days. The biofilm was daily treated using (1 mL/1 min): Vochysia tucanorum Mart. (2.5 mg/mL); Myrcia bella Cambess. (1.25 mg/mL); Matricaria chamomilla L. (20 mg/mL); Malva sylvestris (Malvatricin® Plus–Daudt); 0.12 % Chlorhexidine (PerioGard®-Palmolive, Positive control) and PBS (Negative control). The % dead bacteria, biofilm thickness, EPS biovolume, lactic acid concentration, the CFU counting (total microorganisms, Lactobacillus sp., total streptococci and Streptococcus mutans/S. sobrinus) were determined. Enamel demineralization was measured by TMR. Results: All mouth rinses induced bacterial death compared to PBS (p < 0.0001). The biofilm thickness varied from 12 ± 2 μm (chlorhexidine) to 18 ± 2 μm (V. tucanorum) (ANOVA/Tukey, p < 0.0001). The EPS biovolume varied from 7(4)% (chlorhexidine) to 30(20)% (PBS) (Kruskal-Wallis/Dunn, p < 0.0001). The lactic acid production was reduced by M. sylvestris (1.1 ± 0.2 g/L) and chlorhexidine (0.6 ± 0.2 g/L) compared to PBS (2.6 ± 1.3 g/L) (ANOVA, p < 0.0001). Malva sylvestris and chlorhexidine showed significant low CFU for total microorganisms, Lactobacillus sp. and total streptococci. Only chlorhexidine significantly reduced S. mutans/S. sobrinus. CFUs for total streptococci and Lactobacillus sp, were also significantly reduced by M. chamomilla L. Malva sylvestris (63.4 % of mineral loss reduction), chlorhexidine (47.4 %) and M. chamomilla L. (39.4 %) significantly reduced enamel demineralization compared to PBS (ANOVA/Tukey, p < 0.0001). Conclusion: M. chamomilla L. has lower antibiofilm action, but comparable anti-caries effect to those found for chlorhexidine, under this model. Clinical relevance: This study shows that the antibiofilm and anti-caries potential may vary between the commercial and experimental mouth rinses containing natural agents, with promising results for those containing Matricaria chamomilla L. and Malva Sylvestris.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo-
Descrição: dc.descriptionDepartment of Biological Sciences School of Science The São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biological Sciences School of Science The São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationJournal of Dentistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDental caries-
Palavras-chave: dc.subjectEnamel-
Palavras-chave: dc.subjectMicrocosm biofilm-
Palavras-chave: dc.subjectMouth wash-
Palavras-chave: dc.subjectNatural agents-
Título: dc.titleAntibiofilm and anti-caries effects of an experimental mouth rinse containing Matricaria chamomilla L. extract under microcosm biofilm on enamel-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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