Cisplatin treatment modulates Annexin A1 and inhibitor of differentiation to DNA 1 expression in cervical cancer cells

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorFaceres School of Medicine-
Autor(es): dc.contributorSão José do Rio Preto School of Medicine (FAMERP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorPrates, Janesly [UNESP]-
Autor(es): dc.creatorMoreli, Jusciéle Brogin-
Autor(es): dc.creatorGimenes, Alexandre Dantas-
Autor(es): dc.creatorBiselli, Joice Matos [UNESP]-
Autor(es): dc.creatorPires D'Avila, Solange Correa Garcia-
Autor(es): dc.creatorSandri, Silvana-
Autor(es): dc.creatorFarsky, Sandra Helena Poliselli-
Autor(es): dc.creatorRodrigues-Lisoni, Flávia Cristina [UNESP]-
Autor(es): dc.creatorOliani, Sonia Maria [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:32:43Z-
Data de disponibilização: dc.date.available2022-02-22T00:32:43Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.biopha.2020.110331-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201139-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201139-
Descrição: dc.descriptionCisplatin (Cis) is a choice chemotherapy approach to cervical cancer by inducing DNA adducts and subsequent apoptosis. We have investigated the effects of Cis on Annexin A1 (ANXA1) and inhibitor of DNA binding 1 (ID1) proteins expression to elucidate further mechanisms of Cis actions. Human cervical tissue samples from twenty-four patients, with Cervical Intraepithelial Neoplasia (CIN, stage I, II and III), were evaluated to quantified ANXA1 and ID1 expressions. In vitro, human epidermoid carcinoma of the cervix (SiHa cell line) were treated with Annexin A1 peptide (ANXA12−26), Cis or Cis + ANXA12−26 to evaluate cell proliferation and migration, cytotoxicity of treatments as well as ANXA1 and ID1 modulations by mRNA and protein expression. Our findings showed expression of ID1 and ANXA1 proteins in tissue samples from Cervical Intraepithelial Neoplasia (CIN) patients, with intense immunological identification of ID1 in the CIN III stage. In SiHa cells, treatments with Cis alone or Cis + ANXA12−26, increase mRNA expressions of the ANXA1 and reduced the ID1. In agreement, Cis + ANXA12−26 enhanced ANXA1 protein expression and Cis or Cis + ANXA12−26 abolished ID1 protein expression. Cell proliferation was reduced after treatment with ANXA12−26 peptide and more significant after Cis or Cis + ANXA12−26 treatments. These two last treatments reduced cell viability, by inducing late apoptosis, and impaired cell migration. Together, our data highlight endogenous ANXA1 is involved in Cis therapy for cervical cancer.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (Unesp) Institute of Biosciences Humanities and Exact Sciences (Ibilce), Campus São José do Rio Preto-
Descrição: dc.descriptionUniversidade Federal de São Paulo – UNIFESP Post-Graduation in Structural and Functional Biology-
Descrição: dc.descriptionFaceres School of Medicine, São José do Rio Preto-
Descrição: dc.descriptionDepartment of Pathology São José do Rio Preto School of Medicine (FAMERP), São José do Rio Preto-
Descrição: dc.descriptionSão Paulo University (USP) Department of Clinical and Toxicological Analysis Faculty of Pharmaceutical Sciences-
Descrição: dc.descriptionSão Paulo State University (Unesp) Ilha Solteira School of Engineering (FEIS), Campus Ilha Solteira-
Descrição: dc.descriptionSão Paulo State University (Unesp) Institute of Biosciences Humanities and Exact Sciences (Ibilce), Campus São José do Rio Preto-
Descrição: dc.descriptionSão Paulo State University (Unesp) Ilha Solteira School of Engineering (FEIS), Campus Ilha Solteira-
Descrição: dc.descriptionCNPq: 140883/2014-2-
Descrição: dc.descriptionFAPESP: 2016/02012-4-
Descrição: dc.descriptionCNPq: 308144/2014-7-
Idioma: dc.languageen-
Relação: dc.relationBiomedicine and Pharmacotherapy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectApoptosis-
Palavras-chave: dc.subjectCell proliferation-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectPeptide ANXA12-26-
Palavras-chave: dc.subjectSiHa cells-
Título: dc.titleCisplatin treatment modulates Annexin A1 and inhibitor of differentiation to DNA 1 expression in cervical cancer cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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