Analysis of the Virulence Profile and Phenotypic Features of Typical and Atypical Enteroaggregative Escherichia coli (EAEC) Isolated From Diarrheal Patients in Brazil

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorInstituto Adolfo Lutz-
Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorInstituto Butantan-
Autor(es): dc.creatorDias, Regiane C. B. [UNESP]-
Autor(es): dc.creatorTanabe, Rodrigo H. S. [UNESP]-
Autor(es): dc.creatorVieira, Melissa A. [UNESP]-
Autor(es): dc.creatorCergole-Novella, Maria C.-
Autor(es): dc.creatordos Santos, Luís F.-
Autor(es): dc.creatorGomes, Tânia A. T.-
Autor(es): dc.creatorElias, Waldir P.-
Autor(es): dc.creatorHernandes, Rodrigo T. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:32:39Z-
Data de disponibilização: dc.date.available2022-02-22T00:32:39Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-04-22-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fcimb.2020.00144-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201123-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201123-
Descrição: dc.descriptionEnteroaggregative Escherichia coli (EAEC) is an important agent of acute and persistent diarrhea in children and adults worldwide. Here we report a characterization of 220 EAEC isolates, 88.2% (194/220) of which were typical and 11.8% (26/220) were atypical, obtained from diarrheal patients during seven years (2010-2016) of epidemiological surveillance in Brazil. The majority of the isolates were assigned to phylogroups A (44.1%, 97/220) or B1 (21.4%, 47/220). The aggregative adherence (AA) pattern was detected in 92.7% (204/220) of the isolates, with six of them exhibiting AA concomitantly with a chain-like adherence pattern; and agg5A and agg4A were the most common adhesin-encoding genes, which were equally detected in 14.5% (32/220) of the isolates. Each of 12 virulence factor-encoding genes (agg4A, agg5A, pic, aap, aaiA, aaiC, aaiG, orf3, aar, air, capU, and shf) were statistically associated with typical EAEC (P < 0.05). The genes encoding the newly described aggregate-forming pili (AFP) searched (afpB, afpD, afpP, and afpA2), and/or its regulator (afpR), were exclusively detected in atypical EAEC (57.7%, 15/26), and showed a significant association with this subgroup of EAEC (P < 0.001). In conclusion, we presented an extensive characterization of the EAEC circulating in the Brazilian settings and identified the afp genes as putative markers for increasing the efficiency of atypical EAEC diagnosis.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) Instituto de Biociências-
Descrição: dc.descriptionLaboratório Regional de Santo André Instituto Adolfo Lutz-
Descrição: dc.descriptionCentro de Bacteriologia Instituto Adolfo Lutz-
Descrição: dc.descriptionDepartamento de Microbiologia Imunologia e Parasitologia Universidade Federal de São Paulo- Escola Paulista de Medicina-
Descrição: dc.descriptionLaboratório de Bacteriologia Instituto Butantan-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) Instituto de Biociências-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Cellular and Infection Microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectantimicrobial resistance-
Palavras-chave: dc.subjectdiagnosis-
Palavras-chave: dc.subjectdiarrhea-
Palavras-chave: dc.subjectEAEC-
Palavras-chave: dc.subjectvirulence factors-
Título: dc.titleAnalysis of the Virulence Profile and Phenotypic Features of Typical and Atypical Enteroaggregative Escherichia coli (EAEC) Isolated From Diarrheal Patients in Brazil-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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