Xylella fastidiosa subsp. Pauca strains fb7 and 9a5c from citrus display differential behavior, secretome, and plant virulence

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Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorDavis-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorCitriculture Defense Fund (Fundecitrus)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorde Souza, Jessica Brito-
Autor(es): dc.creatorAlmeida-Souza, Hebréia Oliveira-
Autor(es): dc.creatorZaini, Paulo Adriano-
Autor(es): dc.creatorAlves, Mônica Neli [UNESP]-
Autor(es): dc.creatorde Souza, Aline Gomes-
Autor(es): dc.creatorPierry, Paulo Marques-
Autor(es): dc.creatorda Silva, Aline Maria-
Autor(es): dc.creatorGoulart, Luiz Ricardo-
Autor(es): dc.creatorDandekar, Abhaya M.-
Autor(es): dc.creatorNascimento, Rafael-
Data de aceite: dc.date.accessioned2022-02-22T00:32:31Z-
Data de disponibilização: dc.date.available2022-02-22T00:32:31Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-09-02-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms21186769-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201072-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201072-
Descrição: dc.descriptionXylella fastidiosa colonizes the xylem of various cultivated and native plants worldwide. Citrus production in Brazil has been seriously affected, and major commercial varieties remain susceptible to Citrus Variegated Chlorosis (CVC). Collective cellular behaviors such as biofilm formation influence virulence and insect transmission of X. fastidiosa. The reference strain 9a5c produces a robust biofilm compared to Fb7 that remains mostly planktonic, and both were isolated from symptomatic citrus trees. This work deepens our understanding of these distinct behaviors at the molecular level, by comparing the cellular and secreted proteomes of these two CVC strains. Out of 1017 identified proteins, 128 showed differential abundance between the two strains. Different protein families were represented such as proteases, hemolysin-like proteins, and lipase/esterases, among others. Here we show that the lipase/esterase LesA is among the most abundant secreted proteins of CVC strains as well, and demonstrate its functionality by complementary activity assays. More severe symptoms were observed in Nicotiana tabacum inoculated with strain Fb7 compared to 9a5c. Our results support that systemic symptom development can be accelerated by strains that invest less in biofilm formation and more in plant colonization. This has potential application in modulating the bacterial-plant interaction and reducing disease severity.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniversidade Federal de Uberlândia-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)-
Descrição: dc.descriptionInstitute of Biotechnology Federal University of Uberlandia, Av. Amazonas, Bloco 2E, Campus Umuarama-
Descrição: dc.descriptionDepartment of Plant Sciences College of Agriculture and Environmental Sciences University of California Davis, 1 Shields Ave-
Descrição: dc.descriptionDepartment of Technology School of Agricultural and Veterinary Studies São Paulo State University (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane-
Descrição: dc.descriptionCitriculture Defense Fund (Fundecitrus), Av. Dr. Adhemar Pereira de Barros 201-
Descrição: dc.descriptionDepartment of Biochemistry Institute of Chemistry University of São Paulo, Av. Prof. Lineu Prestes 748-
Descrição: dc.descriptionDepartment of Technology School of Agricultural and Veterinary Studies São Paulo State University (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane-
Descrição: dc.descriptionFAPESP: 11/24091-0-
Descrição: dc.descriptionFAPEMIG: 88881.064973/2014-01-
Formato: dc.format1-20-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectHemolysin-like protein-
Palavras-chave: dc.subjectLC-MS/MS-
Palavras-chave: dc.subjectLipase-
Palavras-chave: dc.subjectPathogenicity-
Palavras-chave: dc.subjectPeptidase-
Palavras-chave: dc.subjectPhytopathogen-
Palavras-chave: dc.subjectPlant-pathogen interaction-
Palavras-chave: dc.subjectSecretome-
Título: dc.titleXylella fastidiosa subsp. Pauca strains fb7 and 9a5c from citrus display differential behavior, secretome, and plant virulence-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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