The combination of coffee compounds attenuates early fibrosis-associated hepatocarcinogenesis in mice: involvement of miRNA profile modulation

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorBarretos Cancer Hospital-
Autor(es): dc.contributorVrije Universiteit Brussel-
Autor(es): dc.contributorUniversity of Minho-
Autor(es): dc.contributor3B's - PT Government Associate Laboratory-
Autor(es): dc.creatorRomualdo, Guilherme Ribeiro [UNESP]-
Autor(es): dc.creatorPrata, Gabriel Bacil [UNESP]-
Autor(es): dc.creatorda Silva, Tereza Cristina-
Autor(es): dc.creatorEvangelista, Adriane Feijó-
Autor(es): dc.creatorReis, Rui Manuel-
Autor(es): dc.creatorVinken, Mathieu-
Autor(es): dc.creatorMoreno, Fernando Salvador-
Autor(es): dc.creatorCogliati, Bruno-
Autor(es): dc.creatorBarbisan, Luís Fernando [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:32:22Z-
Data de disponibilização: dc.date.available2022-02-22T00:32:22Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jnutbio.2020.108479-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/201020-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/201020-
Descrição: dc.descriptionAberrant microRNA expression implicates on hepatocellular carcinoma (HCC) development. Conversely, coffee consumption reduces by ~40% the risk for fibrosis/cirrhosis and HCC, while decaffeinated coffee does not. It is currently unknown whether these protective effects are related to caffeine (CAF), or to its combination with other common and/or highly bioavailable coffee compounds, such as trigonelline (TRI) and chlorogenic acid (CGA). We evaluated whether CAF individually or combined with TRI and/or CGA alleviates fibrosis-associated hepatocarcinogenesis, examining the involvement of miRNA profile modulation. Then, male C3H/HeJ mice were submitted to a diethylnitrosamine/carbon tetrachloride-induced model. Animals received CAF (50 mg/kg), CAF+TRI (50 and 25 mg/kg), CAF+CGA (50 and 25 mg/kg) or CAF+TRI+CGA (50, 25 and 25 mg/kg), intragastrically, 5×/week, for 10 weeks. Only CAF+TRI+CGA combination reduced the incidence, number and proliferation (Ki-67) of hepatocellular preneoplastic foci while enhanced apoptosis (cleaved caspase-3) in adjacent parenchyma. CAF+TRI+CGA treatment also decreased hepatic oxidative stress and enhanced the antioxidant Nrf2 axis. CAF+TRI+CGA had the most pronounced effects on decreasing hepatic pro-inflammatory IL-17 and NFκB, contributing to reduce CD68-positive macrophage number, stellate cell activation, and collagen deposition. In agreement, CAF+TRI+CGA upregulated tumor suppressors miR-144-3p, miR-376a-3p and antifibrotic miR-15b-5p, frequently deregulated in human HCC. CAF+TRI+CGA reduced the hepatic protein levels of pro-proliferative EGFR (miR-144-3p target), antiapoptotic Bcl-2 family members (miR-15b-5p targets), and the number of PCNA (miR-376a-3p target) positive hepatocytes in preneoplastic foci. Our results suggest that the combination of most common and highly bioavailable coffee compounds, rather than CAF individually, attenuates fibrosis-associated hepatocarcinogenesis by modulating miRNA expression profile.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Biosciences Institute São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Pathology School of Veterinary Medicine and Animal Science University of São Paulo (USP)-
Descrição: dc.descriptionMolecular Oncology Research Center Barretos Cancer Hospital-
Descrição: dc.descriptionDepartment of In Vitro Toxicology and Dermato-Cosmetology Faculty of Medicine and Pharmacy Vrije Universiteit Brussel-
Descrição: dc.descriptionDepartment of Food and Experimental Nutrition Faculty of Pharmaceutical Sciences University of São Paulo (USP)-
Descrição: dc.descriptionLife and Health Sciences Research Institute (ICVS) School of Medicine University of Minho-
Descrição: dc.description3B's - PT Government Associate Laboratory-
Descrição: dc.descriptionDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Biosciences Institute São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: #2016/12015–0-
Descrição: dc.descriptionFAPESP: #2016/14420–0-
Descrição: dc.descriptionFAPESP: #2017/16596–0-
Idioma: dc.languageen-
Relação: dc.relationJournal of Nutritional Biochemistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCaffeine-
Palavras-chave: dc.subjectChlorogenic acid-
Palavras-chave: dc.subjectHepatocarcinogenesis-
Palavras-chave: dc.subjectLiver fibrosis-
Palavras-chave: dc.subjectmiRNA-
Palavras-chave: dc.subjectTrigonelline-
Título: dc.titleThe combination of coffee compounds attenuates early fibrosis-associated hepatocarcinogenesis in mice: involvement of miRNA profile modulation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.