Lost and found: Frogs in a biodiversity hotspot rediscovered with environmental DNA

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorSPYGEN-
Autor(es): dc.contributorUniversidade Federal do Rio de Janeiro (UFRJ)-
Autor(es): dc.contributorCornell University-
Autor(es): dc.creatorLopes, Carla Martins [UNESP]-
Autor(es): dc.creatorBaêta, Délio [UNESP]-
Autor(es): dc.creatorValentini, Alice-
Autor(es): dc.creatorLyra, Mariana Lúcio [UNESP]-
Autor(es): dc.creatorSabbag, Ariadne Fares [UNESP]-
Autor(es): dc.creatorGasparini, João Luiz-
Autor(es): dc.creatorDejean, Tony-
Autor(es): dc.creatorHaddad, Célio Fernando Basptista [UNESP]-
Autor(es): dc.creatorZamudio, Kelly Raquel-
Data de aceite: dc.date.accessioned2022-02-22T00:32:16Z-
Data de disponibilização: dc.date.available2022-02-22T00:32:16Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/mec.15594-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200983-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200983-
Descrição: dc.descriptionDeclines and extinctions are increasing globally and challenge conservationists to keep pace with biodiversity monitoring. Organisms leave DNA traces in the environment, e.g., in soil, water, and air. These DNA traces are referred to as environmental DNA (eDNA). The analysis of eDNA is a highly sensitive method with the potential to rapidly assess local diversity and the status of threatened species. We searched for DNA traces of 30 target amphibian species of conservation concern, at different levels of threat, using an environmental DNA metabarcoding approach, together with an extensive sequence reference database to analyse water samples from six montane sites in the Atlantic Coastal Forest and adjacent Cerrado grasslands of Brazil. We successfully detected DNA traces of four declined species (Hylodes ornatus, Hylodes regius, Crossodactylus timbuhy, and Vitreorana eurygnatha); two locally disappeared (Phasmahyla exilis and Phasmahyla guttata); and one species that has not been seen since 1968 (putatively assigned to Megaelosia bocainensis). We confirm the presence of species undetected by traditional methods, underscoring the efficacy of eDNA metabarcoding for biodiversity monitoring at low population densities, especially in megadiverse tropical sites. Our results support the potential application of eDNA in conservation biology, to evaluate persistence and distribution of threatened species in surveyed habitats or sites, and improve accuracy of red lists, especially for species undetected over long periods.-
Descrição: dc.descriptionDepartamento de Biodiversidade e Centro de Aquicultura I.B. Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionSPYGEN-
Descrição: dc.descriptionNúcleo em Ecologia e Desenvolvimento Socioambiental de Macaé Universidade Federal do Rio de Janeiro (UFRJ)-
Descrição: dc.descriptionDepartment of Ecology and Evolutionary Biology Cornell University-
Descrição: dc.descriptionDepartamento de Biodiversidade e Centro de Aquicultura I.B. Universidade Estadual Paulista (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationMolecular Ecology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectamphibians-
Palavras-chave: dc.subjectarchival DNA-
Palavras-chave: dc.subjectbiomonitoring-
Palavras-chave: dc.subjectendangered species-
Palavras-chave: dc.subjectmetabarcoding-
Palavras-chave: dc.subjectpopulation declining-
Título: dc.titleLost and found: Frogs in a biodiversity hotspot rediscovered with environmental DNA-
Aparece nas coleções:Repositório Institucional - Unesp

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