The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorRossoni, Rodnei Dennis [UNESP]-
Autor(es): dc.creatorde Barros, Patrícia Pimentel [UNESP]-
Autor(es): dc.creatorMendonça, Iatã do Carmo [UNESP]-
Autor(es): dc.creatorMedina, Rebeca Previate [UNESP]-
Autor(es): dc.creatorSilva, Dulce Helena Siqueira [UNESP]-
Autor(es): dc.creatorFuchs, Beth Burgwyn-
Autor(es): dc.creatorJunqueira, Juliana Campos [UNESP]-
Autor(es): dc.creatorMylonakis, Eleftherios-
Data de aceite: dc.date.accessioned2022-02-22T00:32:14Z-
Data de disponibilização: dc.date.available2022-02-22T00:32:14Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fcimb.2020.00397-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200968-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200968-
Descrição: dc.descriptionCandida auris has emerged as a medically important pathogen with considerable resistance to antifungal agents. The ability to produce biofilms is an important pathogenicity feature of this species that aids escape of host immune responses and antimicrobial agents. The objective of this study was to verify antifungal action using in vitro and in vivo models of the Lactobacillus paracasei 28.4 probiotic cells and postbiotic activity of crude extract (LPCE) and fraction 1 (LPF1), derived from L. paracasei 28.4 supernatant. Both live cells and cells free supernatant of L. paracasei 28.4 inhibited C. auris suggesting probiotic and postbiotic effects. The minimum inhibitory concentration (MIC) for LPCE was 15 mg/mL and ranges from 3.75 to 7.5 mg/mL for LPF1. Killing kinetics determined that after 24 h treatment with LPCE or LPF1 there was a complete reduction of viable C. auris cells compared to fluconazole, which decreased the initial inoculum by 1-logCFU during the same time period. LPCE and LPF1 significantly reduced the biomass (p = 0.0001) and the metabolic activity (p = 0.0001) of C. auris biofilm. There was also a total reduction (~108 CFU/mL) in viability of persister C. auris cells after treatment with postbiotic elements (p < 0.0001). In an in vivo study, injection of LPCE and LPF1 into G. mellonella larvae infected with C. auris prolonged survival of these insects compared to a control group (p < 0.05) and elicited immune responses by increasing the number of circulating hemocytes and gene expression of antimicrobial peptide galiomicin. We concluded that the L. paracasei 28.4 cells and postbiotic elements (LPCE and LPF1) have antifungal activity against planktonic cells, biofilms, and persister cells of C. auris. Postbiotic supplementation derived from L. paracasei 28.4 protected G. mellonella infected with C. auris and enhanced its immune status indicating a dual function in modulating a host immune response.-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University/UNESP-
Descrição: dc.descriptionDivision of Infectious Diseases, Rhode Island Hospital, Warren Alpert Medical School at Brown University, Providence, RI, United States-
Descrição: dc.descriptionDepartment of Organic Chemistry Center for Bioassays Biosynthesis and Ecophysiology of Natural Products Institute of Chemistry São Paulo State University UNESP-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University/UNESP-
Descrição: dc.descriptionDepartment of Organic Chemistry Center for Bioassays Biosynthesis and Ecophysiology of Natural Products Institute of Chemistry São Paulo State University UNESP-
Formato: dc.format397-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in cellular and infection microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiofilms-
Palavras-chave: dc.subjectCandida auris-
Palavras-chave: dc.subjectLactobacillus-
Palavras-chave: dc.subjectpostbiotic-
Palavras-chave: dc.subjectprobiotic-
Título: dc.titleThe Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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