Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | University of Texas Medical Branch at Galveston | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | de Castro Silva, Mariana [UNESP] | - |
Autor(es): dc.creator | Richardson, Lauren S. | - |
Autor(es): dc.creator | Kechichian, Talar | - |
Autor(es): dc.creator | Urrabaz-Garza, Rheanna | - |
Autor(es): dc.creator | da Silva, Márcia Guimarães [UNESP] | - |
Autor(es): dc.creator | Menon, Ramkumar | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:32:13Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:32:13Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-10-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1530/REP-20-0283 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/200960 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/200960 | - |
Descrição: dc.description | A non-reversible state of epithelial to mesenchymal transition (EMT) at term accumulates proinflammatory mesenchymal cells and predisposes fetal membrane to weakening prior to delivery at term. We investigated the induction of EMT in amnion epithelial cells (AEC) in response to inflammation and infection associated with spontaneous preterm birth (SPTB). For this, membranes from SPTB were screened for EMT markers. Primary AEC in culture were treated with TNF-α (10 and 50 ng/mL) and LPS (50 and 100 ng/mL) for 72 h. Cell shape index (SI) was determined based on morphological shift (microscopy followed by ImageJ software analysis). Immunocytochemistry and Western blot assessed changes in epithelial markers (cytokeratin-18 and E-cadherin) and mesenchymal markers (vimentin and N-cadherin). Involvement of transforming growth factor beta (TGF-β) in EMT induction and EMT associated inflammation was tested using specific markers (Western blot) and by measuring MMP9 (ELISA), respectively. We report that PTB is associated with fetal membrane EMT. TNF-α produced dose- and time-dependent induction of EMT; within 24 h by 50 ng/mL and after 72 h by 10 ng/mL. AEC showed mesenchymal morphology, lower E-cadherin, higher vimentin and N-cadherin and higher MMP9 compared to control. TNF-α-induced EMT was not associated with canonical TGF-β pathway. LPS, regardless of dose or time, did not induce EMT in AEC. We conclude that PTB with intact membranes is associated with EMT. Our data suggest that inflammation, but not infection, is associated with non-canonical activation of EMT and inflammation that can predispose membrane to undergo weakening. | - |
Descrição: dc.description | Division of Maternal-Fetal Medicine and Perinatal Research Department of Obstetrics & Gynecology University of Texas Medical Branch at Galveston | - |
Descrição: dc.description | Department of Pathology Botucatu Medical School Universidade Estadual Paulista UNESP | - |
Descrição: dc.description | Department of Pathology Botucatu Medical School Universidade Estadual Paulista UNESP | - |
Formato: dc.format | 627-638 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Reproduction (Cambridge, England) | - |
???dc.source???: dc.source | Scopus | - |
Título: dc.title | Inflammation, but not infection, induces EMT in human amnion epithelial cells | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: