SIRT1-dependent effects of resveratrol and grape juice in an in vitro model of preeclampsia

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorViana-Mattioli, Sarah [UNESP]-
Autor(es): dc.creatorCinegaglia, Naiara [UNESP]-
Autor(es): dc.creatorBertozzi-Matheus, Mariana [UNESP]-
Autor(es): dc.creatorBueno-Pereira, Thaina Omia [UNESP]-
Autor(es): dc.creatorCaldeira-Dias, Mayara [UNESP]-
Autor(es): dc.creatorCavalli, Ricardo Carvalho-
Autor(es): dc.creatorSandrim, Valeria Cristina [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:32:12Z-
Data de disponibilização: dc.date.available2022-02-22T00:32:12Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.biopha.2020.110659-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200957-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200957-
Descrição: dc.descriptionPreeclampsia (PE) is a multifactorial hypertensive disorder of pregnancy that is partly responsible for both maternal and fetal morbidity and mortality levels worldwide. It has been recently discovered that sirtuin-1 (SIRT1) is reduced in the circulation and in an in vitro model of PE. Therefore, in this study, we investigated the effects of trans-resveratrol, a potent antioxidant and activator of SIRT1, on oxidative stress and nitric oxide (NO) production in an in vitro model of PE compared to gestational hypertensive (GH) and healthy pregnant (HP) women. Furthermore, we also evaluated the effects of an acute intake of grape juice on women with PE to assess whether it could mimic in vitro trans-resveratrol supplementation. (1) In the GH group, resveratrol decreased intracellular reactive oxygen species (ROS) and increased their antioxidant capacity, while inhibiting SIRT1 reestablished previous levels. (2) In PE, inhibition of SIRT1 increased antioxidant activity. (3) Intracellular NO and supernatant nitrite levels were increased by inhibiting SIRT1 in the PE group. (4) Grape juice intake increased intracellular NO levels versus before grape juice intake control; however, the inhibition of SIRT1 before grape juice intake initially increased NO, but decreased it 1 h after grape juice intake. In conclusion, activating SIRT1 by using resveratrol alone may not be beneficial to women with PE, and GH and PE seem to have different responsive mechanisms to this molecule. Furthermore, grape juice intake seems to have different effects compared to resveratrol supplementation alone in this in vitro model of PE, demonstrating the potential of the combination of other biologically active molecules from grape juice over the SIRT1-eNOS-NO in PE treatment.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP) Distrito Rubiao Junior Botucatu-
Descrição: dc.descriptionDepartment of Gynecology and Obstetrics Faculty of Medicine of Ribeirao Preto University of Sao Paulo Ribeirao Preto-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP) Distrito Rubiao Junior Botucatu-
Descrição: dc.descriptionCNPq: 2014-5/305587-
Descrição: dc.descriptionFAPESP: 2015/20461-8-
Descrição: dc.descriptionFAPESP: 2019/06118-0-
Descrição: dc.descriptionFAPESP: 2019/07230-8-
Descrição: dc.descriptionCNPq: 301293/2018-0-
Idioma: dc.languageen-
Relação: dc.relationBiomedicine and Pharmacotherapy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEndothelium-
Palavras-chave: dc.subjectGrape juice-
Palavras-chave: dc.subjectNitric oxide-
Palavras-chave: dc.subjectOxidative stress-
Palavras-chave: dc.subjectPreeclampsia-
Palavras-chave: dc.subjectResveratrol-
Palavras-chave: dc.subjectSIRT1-
Título: dc.titleSIRT1-dependent effects of resveratrol and grape juice in an in vitro model of preeclampsia-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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