Bone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUNOESTE-University of Western Sao Paulo-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Pernambuco (UFPE)-
Autor(es): dc.contributorPrivate Practice-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorSantinoni, Carolina S. [UNESP]-
Autor(es): dc.creatorNeves, Adrieli P. C. [UNESP]-
Autor(es): dc.creatorAlmeida, Breno F. M. [UNESP]-
Autor(es): dc.creatorKajimoto, Natália C. [UNESP]-
Autor(es): dc.creatorPola, Natália M.-
Autor(es): dc.creatorCaliente, Eliana A. [UNESP]-
Autor(es): dc.creatorBelem, Eduarda L. G. [UNESP]-
Autor(es): dc.creatorLelis, Joilson B. [UNESP]-
Autor(es): dc.creatorFucini, Stephen E. [UNESP]-
Autor(es): dc.creatorMessora, Michel R.-
Autor(es): dc.creatorGarcia, Valdir G. [UNESP]-
Autor(es): dc.creatorBomfim, Suely R. M. [UNESP]-
Autor(es): dc.creatorErvolino, Edilson [UNESP]-
Autor(es): dc.creatorNagata, Maria J. H. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:32:05Z-
Data de disponibilização: dc.date.available2022-02-22T00:32:05Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/jbm.a.37076-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200920-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200920-
Descrição: dc.descriptionThe present study evaluated bone marrow aspirate (BMA) and low-level laser therapy (LLLT) on bone healing. It was created critical-size defects (CSD) of 5 mm diameter in rat calvaria of 64 rats. Animals were randomly divided into four groups: Control (blood clot), BMA (coagulated BMA), LLLT (laser irradiation and blood clot), and BMA/LLLT (laser irradiation and coagulated BMA). Euthanasia was performed at 15 or 30 days postoperative. Immunohistochemical reactions were performed to identify vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), runt-related transcription factor-2 (Runx2), bone morphogenetic protein-2 (BMP-2), osteocalcin (OCN), and osteopontin (OPN). The markers were quantified, and data were statistically analyzed. Groups BMA/LLLT and LLLT presented significantly higher VEGF expression than group control. Group BMA/LLLT presented a significantly higher expression of PCNA than all experimental groups. Groups BMA and BMA/LLLT presented significantly higher expression of BMP-2 than all experimental groups. Groups LLLT and BMA/LLLT presented significantly higher expression of OPN than groups control and BMA. Groups LLLT, BMA, and BMA/LLLT presented a significantly higher expression of OCN than group control. It can be concluded that the association of BMA and LLLT enhanced bone healing by improving expression of VEGF, PCNA, Runx2, BMP-2, OPN, and OCN.-
Descrição: dc.descriptionDental School of Presidente Prudente Graduate Program in Dentistry (GPD-Master's Degree) UNOESTE-University of Western Sao Paulo-
Descrição: dc.descriptionDivision of Periodontics Dental School of Araçatuba Univ. Estadual Paulista–UNESP-
Descrição: dc.descriptionDivision of Clinical Surgery and Animal Reproduction Veterinary School of Araçatuba Univ. Estadual Paulista–UNESP-
Descrição: dc.descriptionDivision of Periodontics Dental School of Pelotas Federal University of Pelotas–UFPel-
Descrição: dc.descriptionPeriodontics Private Practice-
Descrição: dc.descriptionDivision of Periodontics School of Dentistry of Ribeirão Preto University of São Paulo-USP-
Descrição: dc.descriptionDivision of Histology Dental School of Araçatuba Univ. Estadual Paulista-UNESP-
Descrição: dc.descriptionDivision of Periodontics Dental School of Araçatuba Univ. Estadual Paulista–UNESP-
Descrição: dc.descriptionDivision of Clinical Surgery and Animal Reproduction Veterinary School of Araçatuba Univ. Estadual Paulista–UNESP-
Descrição: dc.descriptionDivision of Histology Dental School of Araçatuba Univ. Estadual Paulista-UNESP-
Idioma: dc.languageen-
Relação: dc.relationJournal of Biomedical Materials Research - Part A-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbone marrow-
Palavras-chave: dc.subjectbone regeneration-
Palavras-chave: dc.subjectimmunohistochemistry-
Palavras-chave: dc.subjectlow-level laser therapy-
Palavras-chave: dc.subjectmesenchymal stem cells-
Título: dc.titleBone marrow coagulated and low-level laser therapy accelerate bone healing by enhancing angiogenesis, cell proliferation, osteoblast differentiation, and mineralization-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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