Free and Substrate-Immobilised Lipases from Fusarium verticillioides P24 as a Biocatalyst for Hydrolysis and Transesterification Reactions

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorBorges, Janaina Pires [UNESP]-
Autor(es): dc.creatorQuilles Junior, José Carlos [UNESP]-
Autor(es): dc.creatorOhe, Thiago Hideyuki Kobe [UNESP]-
Autor(es): dc.creatorFerrarezi, Ana Lucia [UNESP]-
Autor(es): dc.creatorNunes, Christiane da Costa Carreira [UNESP]-
Autor(es): dc.creatorBoscolo, Mauricio [UNESP]-
Autor(es): dc.creatorGomes, Eleni [UNESP]-
Autor(es): dc.creatorBocchini, Daniela Alonso [UNESP]-
Autor(es): dc.creatorda Silva, Roberto [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:32:04Z-
Data de disponibilização: dc.date.available2022-02-22T00:32:04Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s12010-020-03411-w-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200914-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200914-
Descrição: dc.descriptionFungal enzymes are widely used in technological processes and have some interesting features to be applied in a variety of biosynthetic courses. Here, free and substrate-immobilised lipases from Fusarium verticillioides P24 were obtained by solid-state fermentation using wheat bran as substrate and fungal carrier. Based on their hydrolytic and transesterification activities, the lipases were characterised as pH-dependent in both reactions, with higher substrate conversion in an alkaline environment. Thermally, the lipases performed well from 30 to 45 °C, being more stable in mild conditions. Organic solvents significantly influenced the lipase selectivity using different vegetable oils as fatty acid source. Omega(ω)-3 production in n-hexane achieved 45% using canola oil, against ≈ 18% in cyclohexane. However, ω-6 production was preferably produced for both solvents using linseed oil with significant alterations in the yield (≈ 79% and 49% for n-hexane and cyclohexane, respectively). Moreover, the greatest enzyme selectivity for ω-6 led us to suppose a lipase preference for the Sn1 position of the triacylglycerol. Lastly, a transesterification reaction was performed, achieving 90% of ester conversion in 72 h. This study reports the characterisation and use of free and substrate-immobilised lipases from Fusarium verticillioides P24 as an economic and efficient method for the first time.-
Descrição: dc.descriptionDepartament of Biochemistry and Chemical Technology IQ/UNESP, Rua Prof. Francisco Degni, 55, CEP-
Descrição: dc.descriptionDepartment of Chemistry and Environmental Sciences IBILCE/UNESP, Rua Cristóvão Colombo, 2265, CEP-
Descrição: dc.descriptionDepartment of Biology IBILCE/UNESP, Rua Cristóvão Colombo, 2265, CEP-
Descrição: dc.descriptionDepartament of Biochemistry and Chemical Technology IQ/UNESP, Rua Prof. Francisco Degni, 55, CEP-
Descrição: dc.descriptionDepartment of Chemistry and Environmental Sciences IBILCE/UNESP, Rua Cristóvão Colombo, 2265, CEP-
Descrição: dc.descriptionDepartment of Biology IBILCE/UNESP, Rua Cristóvão Colombo, 2265, CEP-
Idioma: dc.languageen-
Relação: dc.relationApplied Biochemistry and Biotechnology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectFusarium verticillioides-
Palavras-chave: dc.subjectHydrolysis-
Palavras-chave: dc.subjectLipase-
Palavras-chave: dc.subjectOmega-3-
Palavras-chave: dc.subjectOmega-6-
Palavras-chave: dc.subjectTransesterification-
Título: dc.titleFree and Substrate-Immobilised Lipases from Fusarium verticillioides P24 as a Biocatalyst for Hydrolysis and Transesterification Reactions-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.