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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Borges, Janaina Pires [UNESP] | - |
Autor(es): dc.creator | Quilles Junior, José Carlos [UNESP] | - |
Autor(es): dc.creator | Ohe, Thiago Hideyuki Kobe [UNESP] | - |
Autor(es): dc.creator | Ferrarezi, Ana Lucia [UNESP] | - |
Autor(es): dc.creator | Nunes, Christiane da Costa Carreira [UNESP] | - |
Autor(es): dc.creator | Boscolo, Mauricio [UNESP] | - |
Autor(es): dc.creator | Gomes, Eleni [UNESP] | - |
Autor(es): dc.creator | Bocchini, Daniela Alonso [UNESP] | - |
Autor(es): dc.creator | da Silva, Roberto [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:32:04Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:32:04Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2019-12-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1007/s12010-020-03411-w | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/200914 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/200914 | - |
Descrição: dc.description | Fungal enzymes are widely used in technological processes and have some interesting features to be applied in a variety of biosynthetic courses. Here, free and substrate-immobilised lipases from Fusarium verticillioides P24 were obtained by solid-state fermentation using wheat bran as substrate and fungal carrier. Based on their hydrolytic and transesterification activities, the lipases were characterised as pH-dependent in both reactions, with higher substrate conversion in an alkaline environment. Thermally, the lipases performed well from 30 to 45 °C, being more stable in mild conditions. Organic solvents significantly influenced the lipase selectivity using different vegetable oils as fatty acid source. Omega(ω)-3 production in n-hexane achieved 45% using canola oil, against ≈ 18% in cyclohexane. However, ω-6 production was preferably produced for both solvents using linseed oil with significant alterations in the yield (≈ 79% and 49% for n-hexane and cyclohexane, respectively). Moreover, the greatest enzyme selectivity for ω-6 led us to suppose a lipase preference for the Sn1 position of the triacylglycerol. Lastly, a transesterification reaction was performed, achieving 90% of ester conversion in 72 h. This study reports the characterisation and use of free and substrate-immobilised lipases from Fusarium verticillioides P24 as an economic and efficient method for the first time. | - |
Descrição: dc.description | Departament of Biochemistry and Chemical Technology IQ/UNESP, Rua Prof. Francisco Degni, 55, CEP | - |
Descrição: dc.description | Department of Chemistry and Environmental Sciences IBILCE/UNESP, Rua Cristóvão Colombo, 2265, CEP | - |
Descrição: dc.description | Department of Biology IBILCE/UNESP, Rua Cristóvão Colombo, 2265, CEP | - |
Descrição: dc.description | Departament of Biochemistry and Chemical Technology IQ/UNESP, Rua Prof. Francisco Degni, 55, CEP | - |
Descrição: dc.description | Department of Chemistry and Environmental Sciences IBILCE/UNESP, Rua Cristóvão Colombo, 2265, CEP | - |
Descrição: dc.description | Department of Biology IBILCE/UNESP, Rua Cristóvão Colombo, 2265, CEP | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Applied Biochemistry and Biotechnology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Fusarium verticillioides | - |
Palavras-chave: dc.subject | Hydrolysis | - |
Palavras-chave: dc.subject | Lipase | - |
Palavras-chave: dc.subject | Omega-3 | - |
Palavras-chave: dc.subject | Omega-6 | - |
Palavras-chave: dc.subject | Transesterification | - |
Título: dc.title | Free and Substrate-Immobilised Lipases from Fusarium verticillioides P24 as a Biocatalyst for Hydrolysis and Transesterification Reactions | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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