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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Warren Alpert Medical School of Brown University | - |
Autor(es): dc.creator | dos Santos, Jéssica Diane [UNESP] | - |
Autor(es): dc.creator | Fugisaki, Luciana Ruano de Oliveira [UNESP] | - |
Autor(es): dc.creator | Medina, Rebeca Previate [UNESP] | - |
Autor(es): dc.creator | Scorzoni, Liliana [UNESP] | - |
Autor(es): dc.creator | Alves, Mariana de Sá [UNESP] | - |
Autor(es): dc.creator | de Barros, Patrícia Pimentel [UNESP] | - |
Autor(es): dc.creator | Ribeiro, Felipe Camargo [UNESP] | - |
Autor(es): dc.creator | Fuchs, Beth Burgwyn | - |
Autor(es): dc.creator | Mylonakis, Eleftherios | - |
Autor(es): dc.creator | Silva, Dulce Helena Siqueira [UNESP] | - |
Autor(es): dc.creator | Junqueira, Juliana Campos [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:31:49Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:31:49Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-07-15 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.3389/fmicb.2020.01605 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/200826 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/200826 | - |
Descrição: dc.description | In the oral cavity, Candida species form mixed biofilms with Streptococcus mutans, a pathogenic bacterium that can secrete quorum sensing molecules with antifungal activity. In this study, we extracted and fractioned culture filtrate of S. mutans, seeking antifungal agents capable of inhibiting the biofilms, filamentation, and candidiasis by Candida albicans. Active S. mutans UA159 supernatant filtrate components were extracted via liquid-liquid partition and fractionated on a C-18 silica column to resolve S. mutans fraction 1 (SM-F1) and fraction 2 (SM-F2). We found anti-biofilm activity for both SM-F1 and SM-F2 in a dose dependent manner and fungal growth was reduced by 2.59 and 5.98 log for SM-F1 and SM-F2, respectively. The SM-F1 and SM-F2 fractions were also capable of reducing C. albicans filamentation, however statistically significant differences were only observed for the SM-F2 (p = 0.004). SM-F2 efficacy to inhibit C. albicans was confirmed by its capacity to downregulate filamentation genes CPH1, EFG1, HWP1, and UME6. Using Galleria mellonella as an invertebrate infection model, therapeutic treatment with SM-F2 prolonged larvae survival. Examination of the antifungal capacity was extended to a murine model of oral candidiasis that exhibited a reduction in C. albicans colonization (CFU/mL) in the oral cavity when treated with SM-F1 (2.46 log) and SM-F2 (2.34 log) compared to the control (3.25 log). Although both SM-F1 and SM-F2 fractions decreased candidiasis in mice, only SM-F2 exhibited significant quantitative differences compared to the non-treated group for macroscopic lesions, hyphae invasion, tissue lesions, and inflammatory infiltrate. Taken together, these results indicate that the SM-F2 fraction contains antifungal components, providing a promising resource in the discovery of new inhibitors for oral candidiasis. | - |
Descrição: dc.description | Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP) | - |
Descrição: dc.description | Division of Infectious Diseases Rhode Island Hospital Warren Alpert Medical School of Brown University | - |
Descrição: dc.description | Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP) | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Frontiers in Microbiology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | biofilm | - |
Palavras-chave: dc.subject | Candida albicans | - |
Palavras-chave: dc.subject | filamentation | - |
Palavras-chave: dc.subject | oral candidiasis | - |
Palavras-chave: dc.subject | Streptococcus mutans | - |
Título: dc.title | Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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