Ehrlichia spp. and Anaplasma spp. in Xenarthra mammals from Brazil, with evidence of novel ‘Candidatus Anaplasma spp.’

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorICAS-Instituto de Conservação de Animais Silvestres-Projeto Bandeiras e Rodovias-
Autor(es): dc.creatorCalchi, Ana Cláudia [UNESP]-
Autor(es): dc.creatorVultão, Juliana Gaboardi-
Autor(es): dc.creatorAlves, Mario Henrique-
Autor(es): dc.creatorYogui, Débora Regina-
Autor(es): dc.creatorDesbiez, Arnaud Leonard Jean-
Autor(es): dc.creatorDe Santi, Mariele [UNESP]-
Autor(es): dc.creatorSantana, Matheus de Souza [UNESP]-
Autor(es): dc.creatorda Silva, Thiago Merighi Vieira [UNESP]-
Autor(es): dc.creatorWerther, Karin [UNESP]-
Autor(es): dc.creatorTeixeira, Marta Maria Geraldes-
Autor(es): dc.creatorMachado, Rosangela Zacarias [UNESP]-
Autor(es): dc.creatorAndré, Marcos Rogério [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:31:48Z-
Data de disponibilização: dc.date.available2022-02-22T00:31:48Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-020-69263-w-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200820-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200820-
Descrição: dc.descriptionAnaplasmataceae agents are obligatory intracellular Gram-negative α-proteobacteria that are transmitted mostly by arthropod vectors. Although mammals of the Superorder Xenarthra (sloths, anteaters, and armadillos) have been implicated as reservoirs for several zoonotic agents, only few studies have sought to detect Anaplasmataceae agents in this group of mammals. This study aimed to investigate the occurrence and genetic diversity of Anaplasma spp. and Ehrlichia spp. in blood and spleen samples of free-living Xenarthra from four different states in Brazil (São Paulo, Mato Grosso do Sul, Rondônia, and Pará). Nested and conventional PCR screening assays were performed to detect the rrs and dsb genes of Anaplasma spp. and Ehrlichia spp., respectively. The assays were positive in 27.57% (91/330) of the Anaplasma spp. and 24.54% (81/330) of the Ehrlichia spp. Of the 91 positive Anaplasma spp. samples, 56.04% were positive in a conventional PCR assay targeting the 23S–5S intergenic region. Phylogenetic and distance analyses based on the rrs gene allocated Anaplasma sequences from sloths captured in Rondônia and Pará states in a single clade, which was closely related to the A. marginale, A. ovis, and A. capra clades. The sequences detected in southern anteaters from São Paulo were allocated in a clade closely related to sequences of Anaplasma spp. detected in Nasua nasua, Leopardus pardalis, and Cerdocyon thous in Brazil. These sequences were positioned close to A. odocoilei sequences. Genotype analysis corroborated previous findings and demonstrated the circulation of two distinct Anaplasma genotypes in animals from north and southeast Brazil. The first genotype was new. The second was previously detected in N. nasua in Mato Grosso do Sul state. The intergenic region analyses also demonstrated two distinct genotypes of Anaplasma. The sequences detected in Xenarthra from Pará and Rondônia states were closely related to those in A. marginale, A. ovis, and A. capra. Anaplasma spp. sequences detected in Xenarthra from São Paulo and were allocated close to those in A. phagocytophilum. The analyses based on the dsb gene grouped the Ehrlichia spp. sequences with sequences of E. canis (São Paulo, Mato Grosso do Sul, and Pará) and E. minasensis (Rondônia and Pará). The data indicate the occurrence of E. canis and E. minasensis and two possible new Candidatus species of Anaplasma spp. in free-living mammals of the Superorder Xenarthra in Brazil.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionLaboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Júlio de Mesquita Filho (UNESP), Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural-
Descrição: dc.descriptionInstituto de Ciências Biomédicas Universidade de São Paulo (USP)-
Descrição: dc.descriptionICAS-Instituto de Conservação de Animais Silvestres-Projeto Bandeiras e Rodovias-
Descrição: dc.descriptionLaboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Júlio de Mesquita Filho (UNESP), Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona Rural-
Descrição: dc.descriptionFAPESP: 2018/13838-6-
Descrição: dc.descriptionCNPq: 302420/2017-7-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleEhrlichia spp. and Anaplasma spp. in Xenarthra mammals from Brazil, with evidence of novel ‘Candidatus Anaplasma spp.’-
Tipo de arquivo: dc.typelivro digital-
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