Effect of fermented sausages with probiotic Enterococcus faecium CRL 183 on gut microbiota using dynamic colonic model

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorSan Miguel de Tucumán-
Autor(es): dc.contributorSchool of Pharmaceutical Sciences-
Autor(es): dc.creatorRoselino, Mariana Nougalli [UNESP]-
Autor(es): dc.creatorSakamoto, Isabel Kimiko-
Autor(es): dc.creatorTallarico Adorno, Maria Angela-
Autor(es): dc.creatorMárcia Canaan, Josiane Maria [UNESP]-
Autor(es): dc.creatorde Valdez, Graciela Font-
Autor(es): dc.creatorRossi, Elizeu Antonio [UNESP]-
Autor(es): dc.creatorSivieri, Katia-
Autor(es): dc.creatorUmbelino Cavallini, Daniela Cardoso [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:31:46Z-
Data de disponibilização: dc.date.available2022-02-22T00:31:46Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.lwt.2020.109876-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200811-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200811-
Descrição: dc.descriptionConsidering the importance of nutrition in maintaining health conditions, this study is aimed at evaluating the effect of the consumption of a potentially probiotic sausage, with reduction of curing salts and fat, fermented with Enterococcus faecium CRL 183 into the gut microbiota, using the simulator of the human microbial ecosystem (SHIME®). The SHIME® experiment was performed using the probiotic sausage for two weeks. The gut microbiota composition was monitored weekly in all colon vessels by plate counts and denaturing gradient gel electrophoresis (PCR-DGGE). Samples of the colonic contents were evaluated microbiologically by plate counts and denaturing gradient gel electrophoresis (PCR-DGGE) and chemically by the production of SCFA (acetate, propionate and butyrate) and ammonium ions (NH+4). It was observed that the consumption of the potentially probiotic sausage can result in a reduced count of Lactobacillus spp. (colon vessels 3, 4 and 5), Bacteroides spp. (colon vessel 5) and Enterobacteriaceae (colon vessels 4 and 5) and increased levels of ammonium ions. The butyric and propionic acids increased (P < 0.05) while acetic acid decreased (P < 0.05) in treatment period. The PCR-DGGE analysis suggested that probiotic sausage increased the microbiota diversity during the treatment phase. Overall, the findings showed that potentially probiotic sausage represents a healthier alternative of fermented meat, with a positive effect on the microbial diversity and metabolite production.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartamento de Análises Clínicas Sao Paulo State University (UNESP) School of Pharmaceutical Sciences, Rodovia Araraquara Jaú, Km 01 – Campus Ville-
Descrição: dc.descriptionSchool of Engineering of São Carlos University of São Paulo, Sao Paulo-
Descrição: dc.descriptionReference Center for Lactobacilos (CERELA) San Miguel de Tucumán-
Descrição: dc.descriptionPostgraduate Program of Food and Nutrition School of Pharmaceutical Sciences, Rodovia Araraquara Jaú, Km 01 – Campus Ville-
Descrição: dc.descriptionDepartamento de Análises Clínicas Sao Paulo State University (UNESP) School of Pharmaceutical Sciences, Rodovia Araraquara Jaú, Km 01 – Campus Ville-
Descrição: dc.descriptionCAPES: 99999.009550/2014-03-
Idioma: dc.languageen-
Relação: dc.relationLWT-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDGGE-
Palavras-chave: dc.subjectEnterococcus faecium CRL 183-
Palavras-chave: dc.subjectFermented sausage-
Palavras-chave: dc.subjectSCFAs-
Palavras-chave: dc.subjectSHIME-
Título: dc.titleEffect of fermented sausages with probiotic Enterococcus faecium CRL 183 on gut microbiota using dynamic colonic model-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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