Anti-PSMA monoclonal antibody increases the toxicity of paclitaxel carried by carbon nanotubes

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUNOESTE – Oeste Paulista University-
Autor(es): dc.creatorComparetti, Edson José [UNESP]-
Autor(es): dc.creatorRomagnoli, Graziela Gorete [UNESP]-
Autor(es): dc.creatorGorgulho, Carolina Mendonça [UNESP]-
Autor(es): dc.creatorPedrosa, Valber de Albuquerque [UNESP]-
Autor(es): dc.creatorKaneno, Ramon [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:31:30Z-
Data de disponibilização: dc.date.available2022-02-22T00:31:30Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.msec.2020.111254-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200721-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200721-
Descrição: dc.descriptionMultiple-wall carbon nanotubes (CNTs) were functionalized with polyethyleneimine in order to incorporate paclitaxel (PTX), the first line chemotherapeutic agent for prostate cancer. These particles were then covered with antibodies for the prostate-specific membrane antigen (PSMA), to address them to prostate cancer cells. LNCaP prostate cancer cells (PSMA+), HCT-116 and CaCo-2 colon cancer cells (PSMA−), as well as human peripheral monocytes and lymphocytes (PSMA−), were in vitro exposed to fluorescent CNT composites. The interaction/adherence of those composites to target cells was analyzed by fluorescence microscopy and flow cytometry, showing a diffuse interaction of CNTs and CNT-PTX with all cell types. Analysis of cytotoxicity revealed that both prostate (PSMA+) and colorectal cancer cells (PSMA−) were more susceptible to PTX complexed with CNTs than to pure PTX or CNTs alone, while the incorporation of anti-PSMA (CNT-PTX-PSMA) improved the toxicity on LNCaP cells but not on PSMA- targets. No toxicity was observed in human monocytes and lymphocytes but composites induced phenotypical changes in monocytes. Our results demonstrate the feasibility of using anti-PSMA antibody to address drug-loaded CNT to cancer cells as a strategy for improving the effectiveness of antineoplastic agents.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University – UNESP Institute of Biosciences – Department of Chemical and Biological Sciences-
Descrição: dc.descriptionSão Paulo State University – UNESP School of Medicine of Botucatu – Department of Pathology-
Descrição: dc.descriptionUNOESTE – Oeste Paulista University Department of Health Sciences-
Descrição: dc.descriptionSão Paulo State University – UNESP Institute of Biosciences – Department of Chemical and Biological Sciences-
Descrição: dc.descriptionSão Paulo State University – UNESP School of Medicine of Botucatu – Department of Pathology-
Descrição: dc.descriptionCNPq: 140250/2016-6-
Descrição: dc.descriptionFAPESP: 2012/20494-5-
Descrição: dc.descriptionFAPESP: 2014/26032-9-
Idioma: dc.languageen-
Relação: dc.relationMaterials Science and Engineering C-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnti-PSMA-
Palavras-chave: dc.subjectCarbon nanotubes-
Palavras-chave: dc.subjectChemotherapy-
Palavras-chave: dc.subjectNanocarrier-
Palavras-chave: dc.subjectProstate cancer-
Título: dc.titleAnti-PSMA monoclonal antibody increases the toxicity of paclitaxel carried by carbon nanotubes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.