Comparison of generational effect on proteins and metabolites in non-transgenic and transgenic soybean seeds through the insertion of the cp4-EPSPS gene assessed by omics-based platforms

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUK Research and Innovation-
Autor(es): dc.creatorde Campos, Bruna K.-
Autor(es): dc.creatorGalazzi, Rodrigo M.-
Autor(es): dc.creatordos Santos, Bruna M. [UNESP]-
Autor(es): dc.creatorBalbuena, Tiago S. [UNESP]-
Autor(es): dc.creatordos Santos, Fábio N.-
Autor(es): dc.creatorMokochinski, João B.-
Autor(es): dc.creatorEberlin, Marcos N.-
Autor(es): dc.creatorArruda, Marco A.Z.-
Data de aceite: dc.date.accessioned2022-02-22T00:31:29Z-
Data de disponibilização: dc.date.available2022-02-22T00:31:29Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ecoenv.2020.110918-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200712-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200712-
Descrição: dc.descriptionThis work evaluates different generations of transgenic (cp4-EPSPS gene) and non-transgenic soybean plants through proteomics and metabolomics. For proteomics purpose, 24 differentially abundant protein spots were found through 2-D DIGE, being 4 belonging to transgenic plants. From this total, 19 were successfully identified, storage proteins as predominant class. Some identified proteins are involved in growing and cell division, and stress response, such as LEA and dehydrin. For metabolomics, 17 compounds were putatively annotated, mainly belonging to the secondary metabolism, such as flavonoids. From these analyzes, all generations and varieties of the soybean are prone to be differentiate by PLS-DA. According to our results, transgenic plants appear to be more stable than non-transgenic ones. In addition, the omics-based approaches allowed access some relations between those differential spot proteins and metabolites, mainly those storage proteins and flavonoid.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionSpectrometry Sample Preparation and Mechanization Group – GEPAM Institute of Chemistry University of Campinas – UNICAMP, P.O. Box 6154-
Descrição: dc.descriptionNational Institute of Science and Technology for Bioanalytics Institute of Chemistry University of Campinas – UNICAMP, P.O. Box 6154-
Descrição: dc.descriptionDepartment of Technology School of Agricultural and Veterinary Studies State University “Júlio de Mesquita Filho”- UNESP - Jaboticabal-
Descrição: dc.descriptionDepartment of Organic Chemistry Institute of Chemistry University of Campinas – UNICAMP, P.O. Box 6154-
Descrição: dc.descriptionLondon Institute of Medical Sciences Faculty of Medicine Imperial College London UK Research and Innovation-
Descrição: dc.descriptionDepartment of Technology School of Agricultural and Veterinary Studies State University “Júlio de Mesquita Filho”- UNESP - Jaboticabal-
Descrição: dc.descriptionFAPESP: 2013/15646-3-
Descrição: dc.descriptionFAPESP: 2013/17514-7-
Descrição: dc.descriptionFAPESP: 2016/07384-7-
Descrição: dc.descriptionFAPESP: 2018/25207-0-
Descrição: dc.descriptionCNPq: 401170/2016-0-
Idioma: dc.languageen-
Relação: dc.relationEcotoxicology and Environmental Safety-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectGenerational effect-
Palavras-chave: dc.subjectMass spectrometry-
Palavras-chave: dc.subjectMetabolite-
Palavras-chave: dc.subjectProtein-
Palavras-chave: dc.subjectSoybean-
Palavras-chave: dc.subjectTransgenic-
Título: dc.titleComparison of generational effect on proteins and metabolites in non-transgenic and transgenic soybean seeds through the insertion of the cp4-EPSPS gene assessed by omics-based platforms-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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