Functional and mass spectrometric evaluation of an anti-tick antigen based on the P0 peptide conjugated to Bm86 protein

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorCenter for Genetic Engineering and Biotechnology (CIGB)-
Autor(es): dc.contributorCIGB-
Autor(es): dc.contributorPontifícia Universidade Católica do Paraná (PUCPR)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorInstituto de Ciencia Animal (ICA)-
Autor(es): dc.contributorNational Laboratory for Parasitology-
Autor(es): dc.contributorCICESE-
Autor(es): dc.contributorInstitut Pasteur de Montevideo-
Autor(es): dc.contributorInstituto de Investigaciones Biológicas Clemente Estable (IIBCE)-
Autor(es): dc.creatorMallón, Alina Rodríguez-
Autor(es): dc.creatorGonzález, Luis Javier-
Autor(es): dc.creatorGuzmán, Pedro Enrique Encinosa-
Autor(es): dc.creatorBechara, Gervasio Henrique [UNESP]-
Autor(es): dc.creatorSanches, Gustavo Seron [UNESP]-
Autor(es): dc.creatorPousa, Satomy-
Autor(es): dc.creatorCabrera, Gleysin-
Autor(es): dc.creatorCabrales, Ania-
Autor(es): dc.creatorGaray, Hilda-
Autor(es): dc.creatorMejías, Raúl-
Autor(es): dc.creatorÁlvarez, José Raúl López-
Autor(es): dc.creatorSoto, Yamil Bello-
Autor(es): dc.creatorAlmeida, Fabiola-
Autor(es): dc.creatorGuirola, Osmany-
Autor(es): dc.creatorFernández, Rafmary Rodríguez-
Autor(es): dc.creatorCastillo, Alier Fuentes-
Autor(es): dc.creatorMéndez, Luis-
Autor(es): dc.creatorJiménez, Samanta-
Autor(es): dc.creatorLicea-Navarro, Alexei-
Autor(es): dc.creatorPortela, Madelón-
Autor(es): dc.creatorDurán, Rosario-
Autor(es): dc.creatorEstrada, Mario Pablo-
Data de aceite: dc.date.accessioned2022-02-22T00:31:23Z-
Data de disponibilização: dc.date.available2022-02-22T00:31:23Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/pathogens9060513-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200678-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200678-
Descrição: dc.descriptionA synthetic 20 amino acid peptide of the ribosomal protein P0 from ticks, when conjugated to keyhole limpet hemocyanin from Megathura crenulata and used as an immunogen against Rhipicephalus microplus and Rhipicephalus sanguineus s.l. species, has shown efficacies of around 90%. There is also experimental evidence of a high efficacy of this conjugate against Amblyomma mixtum and Ixodes ricinus species, which suggest that this antigen could be a good broad-spectrum anti-tick vaccine candidate. In this study, the P0 peptide (pP0) was chemically conjugated to Bm86 as a carrier protein. SDS-PAGE analysis of this conjugate demonstrated that it is highly heterogeneous in size, carrying from 1 to 18 molecules of pP0 per molecule of Bm86. Forty-nine out of the 54 lysine residues and the N-terminal end of Bm86 were found partially linked to pP0 by using LC-MS/MS analysis and the combination of four different softwares. Several post-translational modifications of Bm86 protein were also identified by mass spectrometry. High immunogenicity and efficacy were achieved when dogs and cattle were vaccinated with the pP0–Bm86 conjugate and challenged with R. sanguineus s.l. and R. microplus, respectively. These results encourage the development of this antigen with promising possibilities as an anti-tick vaccine.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionAnimal Biotechnology Department Center for Genetic Engineering and Biotechnology (CIGB)-
Descrição: dc.descriptionMass Spectrometry Laboratory and GlycoLab Department of Proteomics CIGB-
Descrição: dc.descriptionPontifícia Universidade Católica do Paraná (PUCPR)-
Descrição: dc.descriptionDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV-UNESP)-
Descrição: dc.descriptionSynthetic Peptides Group CIGB-
Descrição: dc.descriptionInstituto de Ciencia Animal (ICA)-
Descrição: dc.descriptionBioinformatic Department CIGB-
Descrição: dc.descriptionNational Laboratory for Parasitology-
Descrição: dc.descriptionDepartamento de Innovación Biomédica CICESE-
Descrição: dc.descriptionUnidad de Bioquímica y Proteómica Analítica Institut Pasteur de Montevideo-
Descrição: dc.descriptionInstituto de Investigaciones Biológicas Clemente Estable (IIBCE)-
Descrição: dc.descriptionDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV-UNESP)-
Formato: dc.format1-26-
Idioma: dc.languageen-
Relação: dc.relationPathogens-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnti-tick vaccine-
Palavras-chave: dc.subjectBm86-
Palavras-chave: dc.subjectChemical conjugation-
Palavras-chave: dc.subjectCross-linked peptides-
Palavras-chave: dc.subjectP0-
Palavras-chave: dc.subjectPeptide-
Título: dc.titleFunctional and mass spectrometric evaluation of an anti-tick antigen based on the P0 peptide conjugated to Bm86 protein-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.