Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual de Campinas (UNICAMP) | - |
Autor(es): dc.contributor | S o Paulo State University | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Fernandes, Lu s Gustavo Romani | - |
Autor(es): dc.creator | Perez-Riverol, Amilcar | - |
Autor(es): dc.creator | Bazon, Murilo Luiz [UNESP] | - |
Autor(es): dc.creator | Abram, D bora Moitinho | - |
Autor(es): dc.creator | Brochetto-Braga, M rcia Regina [UNESP] | - |
Autor(es): dc.creator | Zollner, Ricardode Lima | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:31:09Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:31:09Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-06-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.3390/toxins12060379 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/200595 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/200595 | - |
Descrição: dc.description | Insect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) from Polybia paulista wasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated with Polybia paulista venom extract and the proliferation of CD8+ and CD4+ T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4+ T cells, but not of CD8+ T cells. The cytokine pattern showed a high concentration of IFN- and IL-6, and no significant differences to IL-4, IL-1 and TGF- 1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4+CD25+FoxP3+ and CD4+CD25-FoxP3+ Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4+ T cell subset, suggesting its potential use in wasp venom immunotherapy. | - |
Descrição: dc.description | Laboratory of Translational Immunology Medicine School FCM University of Campinas | - |
Descrição: dc.description | Center of the Study of Social Insects Department of General and Applied Biology Institute of Biosciences of Rio Claro Institute of Biosciences of Rio Claro S o Paulo State University | - |
Descrição: dc.description | Laboratory of Arthropods Molecular Biology IBRC UNESP S o Paulo State University | - |
Descrição: dc.description | Venoms and Venomous Animal Studies Center CEVAP S o Paulo State University | - |
Descrição: dc.description | Laboratory of Arthropods Molecular Biology IBRC UNESP S o Paulo State University | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Toxins | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | CD4+ T cells | - |
Palavras-chave: dc.subject | Hymenoptera Venom Allergy | - |
Palavras-chave: dc.subject | Polybia Paulista | - |
Título: dc.title | Functional profile of antigen specific CD4+ T Cells in the Immune response to phospholipase a1 allergen from polybia paulista Venom | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: