Functional profile of antigen specific CD4+ T Cells in the Immune response to phospholipase a1 allergen from polybia paulista Venom

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Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorS o Paulo State University-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorFernandes, Lu s Gustavo Romani-
Autor(es): dc.creatorPerez-Riverol, Amilcar-
Autor(es): dc.creatorBazon, Murilo Luiz [UNESP]-
Autor(es): dc.creatorAbram, D bora Moitinho-
Autor(es): dc.creatorBrochetto-Braga, M rcia Regina [UNESP]-
Autor(es): dc.creatorZollner, Ricardode Lima-
Data de aceite: dc.date.accessioned2022-02-22T00:31:09Z-
Data de disponibilização: dc.date.available2022-02-22T00:31:09Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/toxins12060379-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200595-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200595-
Descrição: dc.descriptionInsect venom can cause systemic allergic reactions, including anaphylaxis. Improvements in diagnosis and venom immunotherapy (VIT) are based on a better understanding of an immunological response triggered by venom allergens. Previously, we demonstrated that the recombinant phospholipase A1 (rPoly p 1) from Polybia paulista wasp venom induces specific IgE and IgG antibodies in sensitized mice, which recognized the native allergen. Here, we addressed the T cell immune response of rPoly p 1-sensitized BALB/c mice. Cultures of splenocytes were stimulated with Polybia paulista venom extract and the proliferation of CD8+ and CD4+ T cells and the frequency of T regulatory cells (Tregs) populations were assessed by flow cytometry. Cytokines were quantified in cell culture supernatants in ELISA assays. The in vitro stimulation of T cells from sensitized mice induces a significant proliferation of CD4+ T cells, but not of CD8+ T cells. The cytokine pattern showed a high concentration of IFN- and IL-6, and no significant differences to IL-4, IL-1 and TGF- 1 production. In addition, the rPoly p 1 group showed a pronounced expansion of CD4+CD25+FoxP3+ and CD4+CD25-FoxP3+ Tregs. rPoly p 1 sensitization induces a Th1/Treg profile in CD4+ T cell subset, suggesting its potential use in wasp venom immunotherapy.-
Descrição: dc.descriptionLaboratory of Translational Immunology Medicine School FCM University of Campinas-
Descrição: dc.descriptionCenter of the Study of Social Insects Department of General and Applied Biology Institute of Biosciences of Rio Claro Institute of Biosciences of Rio Claro S o Paulo State University-
Descrição: dc.descriptionLaboratory of Arthropods Molecular Biology IBRC UNESP S o Paulo State University-
Descrição: dc.descriptionVenoms and Venomous Animal Studies Center CEVAP S o Paulo State University-
Descrição: dc.descriptionLaboratory of Arthropods Molecular Biology IBRC UNESP S o Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationToxins-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCD4+ T cells-
Palavras-chave: dc.subjectHymenoptera Venom Allergy-
Palavras-chave: dc.subjectPolybia Paulista-
Título: dc.titleFunctional profile of antigen specific CD4+ T Cells in the Immune response to phospholipase a1 allergen from polybia paulista Venom-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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