Lack of lymphocytes impairs macrophage polarization and angiogenesis in diabetic wound healing

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Coimbra-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorInstitut Pasteur-
Autor(es): dc.contributorInstitut National de la Santé et de la Recherche Médicale (INSERM) U1223-
Autor(es): dc.contributorFederal University of Paraná-
Autor(es): dc.contributorThe Portuguese Diabetes Association (APDP)-
Autor(es): dc.contributorUniversity of Arkansas for Medical Sciences-
Autor(es): dc.contributorArkansas Children's Research Institute-
Autor(es): dc.contributorUniversity of Porto-
Autor(es): dc.creatorSeraphim, Patricia M. [UNESP]-
Autor(es): dc.creatorLeal, Ermelindo C.-
Autor(es): dc.creatorMoura, João-
Autor(es): dc.creatorGonçalves, Pedro-
Autor(es): dc.creatorGonçalves, Jenifer P.-
Autor(es): dc.creatorCarvalho, Eugénia-
Data de aceite: dc.date.accessioned2022-02-22T00:31:01Z-
Data de disponibilização: dc.date.available2022-02-22T00:31:01Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2020.117813-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200545-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200545-
Descrição: dc.descriptionAIMS: This study aimed to investigate the effect of lymphocytes in wound healing and the underlying mechanisms, in diabetic and non-diabetic mice, using Balb/c recombination activating gene (Rag)-2 and interleukin 2 receptor gamma (IL-2Rγ) double knockout (KO) (RAG2−/− IL-2Rγ−/−) mice. MAIN METHODS: Wound healing in vivo was performed in control and STZ-induced diabetic mice, in both KO and WT mice. Inflammation and ROS production were evaluated by immunofluorescence microscopy analysis, antioxidant enzymes and angiogenesis were evaluated by quantitative PCR and immunofluorescence microscopy analysis, and wound closure kinetics evolution was evaluated by measurement of acetate tracing of the wound area. KEY FINDINGS: Wound closure was significantly delayed in KO mice, where the M1/M2 macrophage ratio and basal ROS levels were significantly increased, while antioxidant defenses and angiogenesis were significantly decreased. Moreover, the expected increase in matrix metallopeptidase (MMP)-9 protein levels in diabetic conditions was not observed in KO mice, suggesting that the mechanisms leading to the increase in MMP-9 observed in diabetic wounds may in part be lymphocyte-dependent. SIGNIFICANCE: Our results indicate that lack of lymphocytes compromises wound healing independent of diabetes. The lack of these cells, even in non-diabetic mice, mimics the phenotype observed in wounds under diabetic conditions. Moreover, the combination of diabetes and the lack of lymphocytes, further impair the wound healing conditions, indicating that when the innate regulatory function is lost in these KO mice, excessive M1 polarization, poor angiogenesis and impaired wound healing are worsen.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCenter for Neuroscience and Cell Biology University of Coimbra-
Descrição: dc.descriptionDepartment of Physiotherapy School of Sciences and Technology Sao Paulo State University - UNESP, Campus Presidente Prudente-
Descrição: dc.descriptionInstituto de Investigação Interdisciplinar University of Coimbra-
Descrição: dc.descriptionInnate Immunity Unit Institut Pasteur-
Descrição: dc.descriptionInstitut National de la Santé et de la Recherche Médicale (INSERM) U1223-
Descrição: dc.descriptionCell Biology Department Federal University of Paraná-
Descrição: dc.descriptionThe Portuguese Diabetes Association (APDP)-
Descrição: dc.descriptionDepartment of Geriatrics University of Arkansas for Medical Sciences-
Descrição: dc.descriptionArkansas Children's Research Institute-
Descrição: dc.descriptionINEB - Instituto Nacional de Engenharia Biomédica University of Porto-
Descrição: dc.descriptioni3S - Instituto de Investigação e Inovação em Saúde University of Porto-
Descrição: dc.descriptionDepartment of Physiotherapy School of Sciences and Technology Sao Paulo State University - UNESP, Campus Presidente Prudente-
Descrição: dc.descriptionCNPq: 203179/2011-0-
Descrição: dc.descriptionCNPq: 233621/2014-8-
Idioma: dc.languageen-
Relação: dc.relationLife Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDiabetes-
Palavras-chave: dc.subjectInflammation-
Palavras-chave: dc.subjectLymphocytes-
Palavras-chave: dc.subjectReactive oxygen species-
Palavras-chave: dc.subjectWound healing-
Título: dc.titleLack of lymphocytes impairs macrophage polarization and angiogenesis in diabetic wound healing-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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