Metalloproteomic Strategies for Identifying Proteins as Biomarkers of Mercury Exposure in Serrasalmus rhombeus from the Amazon Region

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorde Queiroz, João Vitor [UNESP]-
Autor(es): dc.creatorCavecci-Mendonça, Bruna [UNESP]-
Autor(es): dc.creatorVieira, José Cavalcante Souza [UNESP]-
Autor(es): dc.creatorMartins, Renata Aparecida [UNESP]-
Autor(es): dc.creatorde Almeida Assunção, Andrey Sávio [UNESP]-
Autor(es): dc.creatorCavallini, Nubya Gonçalves [UNESP]-
Autor(es): dc.creatordos Santos, Felipe André [UNESP]-
Autor(es): dc.creatorde Magalhães Padilha, Pedro [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:30:53Z-
Data de disponibilização: dc.date.available2022-02-22T00:30:53Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s12011-020-02178-9-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200515-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200515-
Descrição: dc.descriptionThis manuscript describes the results of a metalloproteomic study of mercury in samples of muscle and liver tissue of the species Serrasalmus rhombeus, popularly known as black piranha and characterised as the most voracious and aggressive predator in the Brazilian Amazon. The metalloproteomic study involved using two-dimensional electrophoresis (2D PAGE) to fractionate the proteome of the muscle and liver tissue samples, along with atomic absorption spectrometry in a graphite furnace (GFAAS) to identify mercury associated with protein SPOTs and mass spectrometry with electrospray ionisation (ESI-MS/MS) to characterise the mercury-binding proteins. The protein SPOTs characterised showed concentrations in the order of 156 mg kg−1, which ranks as the highest concentrations of mercury determined so far in metalloproteomic studies involving fish species in the Amazon region. Based on FASTA sequences of proteins characterised by ESI-MS/MS, bioinformatics studies were performed that allowed identifying nine proteins with characteristics of biomarkers of mercury exposure. Of those proteins, glutathione peroxidase stands out as an enzyme of great importance in the antioxidant defence of organisms subjected to oxidative stress caused by xenobiotics.-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP), Rua Prof R. Dr. Valter Maurício Corrêa, s/n-
Descrição: dc.descriptionVenom and Venomous Animals Study Center (CEVAP) of São Paulo State University (UNESP), Rua José Barbosa de Barros, 1780 - Vila Ema-
Descrição: dc.descriptionInstitute of Biosciences São Paulo State University (UNESP), Rua Prof. Dr. Antonio Celso Wagner Zanin, 250-
Descrição: dc.descriptionFaculty of Science and Engineering São Paulo State University (UNESP), R. Domingos da Costa Lopes, 780 - Jardim Itaipu-
Descrição: dc.descriptionSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP), Rua Prof R. Dr. Valter Maurício Corrêa, s/n-
Descrição: dc.descriptionVenom and Venomous Animals Study Center (CEVAP) of São Paulo State University (UNESP), Rua José Barbosa de Barros, 1780 - Vila Ema-
Descrição: dc.descriptionInstitute of Biosciences São Paulo State University (UNESP), Rua Prof. Dr. Antonio Celso Wagner Zanin, 250-
Descrição: dc.descriptionFaculty of Science and Engineering São Paulo State University (UNESP), R. Domingos da Costa Lopes, 780 - Jardim Itaipu-
Idioma: dc.languageen-
Relação: dc.relationBiological Trace Element Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject2D PAGE-
Palavras-chave: dc.subjectESI-MS/MS-
Palavras-chave: dc.subjectExposure biomarkers of Mercury-
Palavras-chave: dc.subjectGFAAS-
Palavras-chave: dc.subjectMercury in Amazon fish-
Palavras-chave: dc.subjectMetallomic studies-
Título: dc.titleMetalloproteomic Strategies for Identifying Proteins as Biomarkers of Mercury Exposure in Serrasalmus rhombeus from the Amazon Region-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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