RAP1-RAC1 Signaling Has an Important Role in Adhesion and Migration in HNSCC

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Michigan School of Dentistry-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Michigan-
Autor(es): dc.creatorLiu, M.-
Autor(es): dc.creatorBanerjee, R.-
Autor(es): dc.creatorRossa, C. [UNESP]-
Autor(es): dc.creatorD’Silva, N. J.-
Data de aceite: dc.date.accessioned2022-02-22T00:30:40Z-
Data de disponibilização: dc.date.available2022-02-22T00:30:40Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1177/0022034520917058-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200439-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200439-
Descrição: dc.descriptionCell-cell adhesion is a key mechanism to control tissue integrity and migration. In head and neck squamous cell carcinoma (HNSCC), cell migration facilitates distant metastases and is correlated with poor prognosis. RAP1, a ras-like protein, has an important role in the progression of HNSCC. RAC1 is an integrin-linked, ras-like protein that promotes cell migration. Here we show that loss of cell-cell adhesion is correlated with inactivation of RAP1 confirmed by 2 different biochemical approaches. RAP1 activation is required for cell-matrix adhesion confirmed by adhesion to fibronectin-coated plates with cells that have biochemically activated RAP1. This effect is reversed when RAP1 is inactivated. In addition, RAP1GTP-mediated adhesion is only facilitated through α5β1 integrin complex and is not a function of either α5 or β1 integrin alone. Moreover, the inside-out signaling of RAP1 activation is coordinated with RAC1 activation. These findings show that RAP1 has a prominent role in cell-matrix adhesion via extracellular matrix molecule fibronectin-induced α5β1 integrin and supports a critical role for the RAP1/RAC1 signaling axis in HNSCC cell migration.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionNational Institute of Dental and Craniofacial Research-
Descrição: dc.descriptionDepartment of Periodontics and Oral Medicine University of Michigan School of Dentistry-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara UNESP—Univ Estadual Paulista-
Descrição: dc.descriptionDepartment of Pathology Medical School University of Michigan-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara UNESP—Univ Estadual Paulista-
Descrição: dc.descriptionFAPESP: 2014/50312-4-
Descrição: dc.descriptionFAPESP: 2017/14283-5-
Descrição: dc.descriptionNational Institute of Dental and Craniofacial Research: DE022567-
Descrição: dc.descriptionNational Institute of Dental and Craniofacial Research: DE027551-
Formato: dc.format959-968-
Idioma: dc.languageen-
Relação: dc.relationJournal of Dental Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcal adhesion kinase-
Palavras-chave: dc.subjectextracellular matrix-
Palavras-chave: dc.subjectfibronectin-
Palavras-chave: dc.subjectintegrin α5β1-
Palavras-chave: dc.subjectsmall GTPases-
Palavras-chave: dc.subjectsquamous cell carcinoma-
Título: dc.titleRAP1-RAC1 Signaling Has an Important Role in Adhesion and Migration in HNSCC-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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