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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | University of Florida | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | University of Florida Health Science Center | - |
Autor(es): dc.contributor | Federal University of Goias (UFG) | - |
Autor(es): dc.contributor | University of North Carolina | - |
Autor(es): dc.creator | Rocha, Fernanda R G. [UNESP] | - |
Autor(es): dc.creator | Delitto, Andrea E. | - |
Autor(es): dc.creator | de Souza, Joao A. Chaves | - |
Autor(es): dc.creator | González-Maldonado, Laura A. [UNESP] | - |
Autor(es): dc.creator | Wallet, Shannon M. | - |
Autor(es): dc.creator | Rossa Junior, Carlos [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:30:25Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:30:25Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-11-30 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1038/s41598-020-64685-y | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/200378 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/200378 | - |
Descrição: dc.description | This study investigates the role of NLRP3 inflammasome and its main effector Caspase-1 in inflammation and alveolar bone resorption associated with periodontitis. Heat-killed Aggregatibacter actinomycetemcomitans (Aa) was injected 3x/week (4 weeks) into gingival tissues of wild-type (WT), Nlrp3-KO and Caspase1-KO mice. Bone resorption was measured by µCT and osteoclast number was determined by tartrate-resistant acid phosphatase (TRAP) staining. Inflammation was assessed histologically (H/E staining and immunofluorescence of CD45 and Ly6G). In vitro studies determined the influence of Nlrp3 and Caspase-1 in Rankl-induced osteoclast differentiation and activity and on LPS-induced expression of inflammation-associated genes. Bone resorption was significantly reduced in Casp1-KO but not in Nlrp3-KO mice. Casp1-KO mice had increased in osteoclast numbers, whereas the inflammatory infiltrate or on gene expression were similar to those of WT and Nlrp3-KO mice. Strikingly, osteoclasts differentiated from Nlrp3-deficient macrophages had increased resorbing activity in vitro. LPS-induced expression of Il-10, Il-12 and Tnf-α was significantly reduced in Nlrp3- and Casp1-deficient macrophages. As an inceptive study, these results suggest that Nlrp3 inflammasome does not play a significant role in inflammation and bone resorption in vivo and that Caspase-1 has a pro-resorptive role in experimental periodontal disease. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | - |
Descrição: dc.description | Department of Oral Biology College of Dentistry University of Florida | - |
Descrição: dc.description | Department of Diagnosis and Surgery UNESP-State University of Sao Paulo School of Dentistry at Araraquara | - |
Descrição: dc.description | Department of Physical Therapy University of Florida Health Science Center | - |
Descrição: dc.description | Department of Stomatology School of Dentistry Federal University of Goias (UFG) | - |
Descrição: dc.description | Department of Oral and Craniofacial Health Sciences School of Dentistry University of North Carolina | - |
Descrição: dc.description | Department of Diagnosis and Surgery UNESP-State University of Sao Paulo School of Dentistry at Araraquara | - |
Descrição: dc.description | FAPESP: 2014/04926-8 | - |
Descrição: dc.description | FAPESP: 2014/17544-6 | - |
Descrição: dc.description | CAPES: AUXPE 655/2014 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Scientific Reports | - |
???dc.source???: dc.source | Scopus | - |
Título: dc.title | Relevance of Caspase-1 and Nlrp3 Inflammasome on Inflammatory Bone Resorption in A Murine Model of Periodontitis | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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