The role of apoptosis associated speck-like protein containing a caspase-1 recruitment domain (ASC) in response to bone substitutes

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Autor(es): dc.contributorUniversidade Veiga de Almeida-
Autor(es): dc.contributorUniversidade Federal Fluminense (UFF)-
Autor(es): dc.contributorCentro Brasileiro de Pesquisas Físicas CBPF-
Autor(es): dc.contributorUniversity of L'Aquila-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorQualidade e Tecnologia (INMETRO)-
Autor(es): dc.contributorUniversidade Iguaçu-
Autor(es): dc.creatorSartoretto, Suelen C.-
Autor(es): dc.creatorCalasans-Maia, Monica D.-
Autor(es): dc.creatorAlves, Adriana T.N.N.-
Autor(es): dc.creatorResende, Rodrigo F.B.-
Autor(es): dc.creatorda Costa Fernandes, Célio Junior [UNESP]-
Autor(es): dc.creatorde Magalhães Padilha, Pedro [UNESP]-
Autor(es): dc.creatorRossi, Alexandre M.-
Autor(es): dc.creatorTeti, Anna-
Autor(es): dc.creatorGranjeiro, José M.-
Autor(es): dc.creatorZambuzzi, Willian F. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:30:04Z-
Data de disponibilização: dc.date.available2022-02-22T00:30:04Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.msec.2020.110965-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200283-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200283-
Descrição: dc.descriptionThe apoptosis-associated Speck-like protein containing a caspase-1 recruitment domain (ASC), present in inflammasomes, regulates inflammation events and is involved in osteogenic phenotype. Nevertheless, its function in bone repair induced by bone substitute biomaterials is unclear. This study aimed to unveil the role of ASC on osteoprogenitor and tissue response to stoichiometric-hydroxyapatite (HA), nanostructured carbonated-hydroxyapatite (CHA), and CHA containing 5% Strontium (SrCHA), characterized previously by XRD, uXRF-SR, and FTIR spectroscopy implants. Thereafter, conditioned media by the biomaterials were used later to treat pre-osteoblasts and an osteogenic stimulus was shown in response to the materials, with higher expression of Runx2, Osterix, ALP, and Collagen 1a1 genes, with significant involvement of inflammatory-related genes. Thus, to better address the involvement of inflammasome, primary cells obtained from both genotypes [Wild-Type (WT) and ASC Knockout (ASC-KO) mice] were subjected to conditioned media up to 7 days, and our data reinforces both HA and CHA induces lower levels of alkaline phosphatase (ALP) than SrCHA, considering both genotypes (p < 0.01), and ASC seems contribute with osteogenic stimulus promoted by SrCHA. Complimentarily, the biomaterials were implanted into both subcutaneous and bone defects in tibia. Histological analysis on 28 days after implantation of biomaterials into mice's subcutaneous tissue revealed moderate inflammatory response to them. Both histomorphometry and μCT analysis of tibias indicated that the biomaterials did not reverse the delay in bone repair of ASC KO, reinforcing the involvement of ASC on bone regeneration and bone de novo deposition. Also, the bone density in CHA was >2-fold higher in WT than ASC-KO samples. HA was virtually not resorbed throughout the experimental periods, in opposition to CHA in the WT group. CHA reduced to half-area after 28 days, and the bone deposition was higher in CHA for WT mice than HA. Taken together, our results show that biomaterials did not interfere with the healing pattern of the ASC KO, but CHA promoted higher bone deposition in the WT group, probably due to its greater biodegradability. These results reinforce the importance of ASC during bone de novo deposition and healing.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)-
Descrição: dc.descriptionOral Surgery Department Universidade Veiga de Almeida-
Descrição: dc.descriptionOral Surgery Department and Clinical Research Laboratory in Dentistry Universidade Federal Fluminense-
Descrição: dc.descriptionOral Diagnosis Department Universidade Federal Fluminense-
Descrição: dc.descriptionDepartment of Applied Physics Centro Brasileiro de Pesquisas Físicas CBPF-
Descrição: dc.descriptionDepartment of Biotechnological and Applied Clinical Sciences University of L'Aquila-
Descrição: dc.descriptionUNESP – São Paulo State University Institute of Biosciences of Botucatu Department of Chemistry and Biochemistry-
Descrição: dc.descriptionClinical Research Laboratory in Dentistry Universidade Federal Fluminense-
Descrição: dc.descriptionDirectory of Life Sciences Applied Metrology Instituto Nacional de Metrologia Qualidade e Tecnologia (INMETRO)-
Descrição: dc.descriptionOral Surgery Department Universidade Iguaçu-
Descrição: dc.descriptionUNESP – São Paulo State University Institute of Biosciences of Botucatu Department of Chemistry and Biochemistry-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPERJ: 2014/22689-3-
Idioma: dc.languageen-
Relação: dc.relationMaterials Science and Engineering C-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectASC-
Palavras-chave: dc.subjectBiomaterials-
Palavras-chave: dc.subjectBone repair-
Palavras-chave: dc.subjectHydroxyapatite-
Palavras-chave: dc.subjectInflammasome-
Título: dc.titleThe role of apoptosis associated speck-like protein containing a caspase-1 recruitment domain (ASC) in response to bone substitutes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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