Blocking drug-induced autophagy with chloroquine in HCT-116 colon cancer cells enhances DC maturation and T cell responses induced by tumor cell lysate

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Pittsburgh-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorZamame Ramirez, Jofer Andree [UNESP]-
Autor(es): dc.creatorRomagnoli, Graziela Gorete [UNESP]-
Autor(es): dc.creatorFalasco, Bianca Francisco [UNESP]-
Autor(es): dc.creatorGorgulho, Carolina Mendonça [UNESP]-
Autor(es): dc.creatorSanzochi Fogolin, Carla [UNESP]-
Autor(es): dc.creatordos Santos, Daniela Carvalho [UNESP]-
Autor(es): dc.creatorJunior, João Pessoa Araújo [UNESP]-
Autor(es): dc.creatorLotze, Michael Thomas-
Autor(es): dc.creatorUreshino, Rodrigo Portes-
Autor(es): dc.creatorKaneno, Ramon [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:29:59Z-
Data de disponibilização: dc.date.available2022-02-22T00:29:59Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.intimp.2020.106495-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200260-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200260-
Descrição: dc.descriptionAutophagy is an important mechanism for tumor escape, allowing tumor cells to recover from the damage induced by chemotherapy, radiation therapy, and immunotherapy and contributing to the development of resistance. The pharmacological inhibition of autophagy contributes to increase the efficacy of antineoplastic agents. Exposing tumor cells to low concentrations of select autophagy-inducing antineoplastic agents increases their immunogenicity and enhances their ability to stimulate dendritic cell (DC) maturation. We tested whether the application of an autophagy-inhibiting agent, chloroquine (CQ), in combination with low concentrations of 5-fluorouracil (5-FU) increases the ability of tumor cells to induce DC maturation. DCs sensitized with the lysate of HCT-116 cells previously exposed to such a combination enhanced the DC maturation/activation ability. These matured DCs also increased the allogeneic responsiveness of both CD4+ and CD8+ T cells, which showed a greater proliferative response than those from DCs sensitized with control lysates. The T cells expanded in such cocultures were CD69+ and PD-1- and produced higher levels of IFN-γ and lower levels of IL-10, consistent with the preferential activation of Th1 cells. Cocultures of autologous DCs and lymphocytes improved the generation of cytotoxic T lymphocytes, as assessed by the expression of CD107a, perforin, and granzyme B. The drug combination increased the expression of genes related to the CEACAM family (BECN1, ATGs, MAPLC3B, ULK1, SQSTM1) and tumor suppressors (PCBP1). Furthermore, the decreased expression of genes related to metastasis and tumor progression (BNIP3, BNIP3L, FOSL2, HES1, LAMB3, LOXL2, NDRG1, P4HA1, PIK3R2) was noted. The combination of 5-FU and CQ increases the ability of tumor cells to drive DC maturation and enhances the ability of DCs to stimulate T cell responses.-
Descrição: dc.descriptionHigher College of Technology-
Descrição: dc.descriptionSão Paulo State University – UNESP Department of Chemical and Biological Sciences Institute of Biosciences of Botucatu-
Descrição: dc.descriptionSão Paulo State University – UNESP Department of Pathology School of Medicine of Botucatu-
Descrição: dc.descriptionSão Paulo State University – UNESP Center for Electron Microscopy Institute of Biosciences of Botucatu-
Descrição: dc.descriptionDepartment of Immunology University of Pittsburgh-
Descrição: dc.descriptionFederal University of São Paulo – UNIFESP Department of Biological Sciences-
Descrição: dc.descriptionSão Paulo State University – UNESP Department of Chemical and Biological Sciences Institute of Biosciences of Botucatu-
Descrição: dc.descriptionSão Paulo State University – UNESP Department of Pathology School of Medicine of Botucatu-
Descrição: dc.descriptionSão Paulo State University – UNESP Center for Electron Microscopy Institute of Biosciences of Botucatu-
Descrição: dc.descriptionHigher College of Technology: CEACAM 5-
Idioma: dc.languageen-
Relação: dc.relationInternational Immunopharmacology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAutophagy-
Palavras-chave: dc.subjectCancer-
Palavras-chave: dc.subjectChemotherapy-
Palavras-chave: dc.subjectColorectal-
Palavras-chave: dc.subjectCytotoxic T cells-
Palavras-chave: dc.subjectDendritic cells-
Título: dc.titleBlocking drug-induced autophagy with chloroquine in HCT-116 colon cancer cells enhances DC maturation and T cell responses induced by tumor cell lysate-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.