The antioxidant peptide salamandrin-i: First bioactive peptide identified from skin secretion of salamandra genus (salamandra salamandra)

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Porto-
Autor(es): dc.contributorIBMC-
Autor(es): dc.contributorUniversity of Brasilia-
Autor(es): dc.contributorUniversity of Brasília-
Autor(es): dc.contributorINEB-
Autor(es): dc.contributorScience and Technology of São Paulo-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorPlácido, Alexandra-
Autor(es): dc.creatorBueno, João-
Autor(es): dc.creatorBarbosa, Eder A.-
Autor(es): dc.creatorMoreira, Daniel C.-
Autor(es): dc.creatorDias, Jhones Do Nascimento-
Autor(es): dc.creatorCabral, Wanessa Felix-
Autor(es): dc.creatorAlbuquerque, Patrícia-
Autor(es): dc.creatorBessa, Lucinda J.-
Autor(es): dc.creatorFreitas, Jaime-
Autor(es): dc.creatorKuckelhaus, Selma A. S.-
Autor(es): dc.creatorLima, Filipe C. D. A.-
Autor(es): dc.creatorBatagin-Neto, Augusto [UNESP]-
Autor(es): dc.creatorBrand, Guilherme D.-
Autor(es): dc.creatorRelvas, João B.-
Autor(es): dc.creatorLeite, José Roberto S. A.-
Autor(es): dc.creatorEaton, Peter-
Data de aceite: dc.date.accessioned2022-02-22T00:29:51Z-
Data de disponibilização: dc.date.available2022-02-22T00:29:51Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/biom10040512-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200233-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200233-
Descrição: dc.descriptionAmphibian skin is a multifunctional organ that plays key roles in defense, breathing, and water balance. In this study, skin secretion samples of the fire salamander (Salamandra salamandra) were separated using RP-HPLC and de novo sequenced using MALDI-TOF MS/MS. Next, we used an in silico platform to screen antioxidant molecules in the framework of density functional theory. One of the identified peptides, salamandrin-I, [M + H]+ = 1406.6 Da, was selected for solid-phase synthesis; it showed free radical scavenging activity against DPPH and ABTS radicals. Salamandrin-I did not show antimicrobial activity against Gram-positive and-negative bacteria. In vitro assays using human microglia and red blood cells showed that salamandrin-I has no cytotoxicity up to the concentration of 100 µM. In addition, in vivo toxicity tests on Galleria mellonella larvae resulted in no mortality at 20 and 40 mg/kg. Antioxidant peptides derived from natural sources are increasingly attracting interest. Among several applications, these peptides, such as salamandrin-I, can be used as templates in the design of novel antioxidant molecules that may contribute to devising strategies for more effective control of neurological disease.-
Descrição: dc.descriptionUniversidade Estadual Paulista-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia-
Descrição: dc.descriptionLAQV/REQUIMTE Department of Chemistry and Biochemistry Faculty of Sciences University of Porto-
Descrição: dc.descriptionInstitute for Research and Innovation in Health (i3S) and Institute for Molecular and Cell Biology IBMC-
Descrição: dc.descriptionCenter for Research in Applied Morphology and Immunology NuPMIA University of Brasilia, DF-
Descrição: dc.descriptionLaboratory for the Synthesis and Analysis of Biomolecules (LSAB) Institute of Chemistry University of Brasília, DF-
Descrição: dc.descriptionLaboratory of Molecular Biology of Fungal Pathogens Department of Cell Biology Institute of Biological Sciences University of Brasília, DF-
Descrição: dc.descriptionInstitute for Research and Innovation in Health (i3S) and National Institute of Biomedical Engineering INEB-
Descrição: dc.descriptionFederal Institute of Education Science and Technology of São Paulo, Campus Matão-
Descrição: dc.descriptionSão Paulo State University (UNESP), Campus of Itapeva-
Descrição: dc.descriptionSão Paulo State University (UNESP), Campus of Itapeva-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionCNPq: 420449/2018-3-
Descrição: dc.descriptionCNPq: 428211/2018-6-
Descrição: dc.descriptionFundação para a Ciência e a Tecnologia: PTDC/BII_BIO/31158/2017-
Idioma: dc.languageen-
Relação: dc.relationBiomolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntioxidant peptides-
Palavras-chave: dc.subjectBioactive molecules-
Palavras-chave: dc.subjectPortuguese biodiversity-
Palavras-chave: dc.subjectSalamandra salamandra-
Título: dc.titleThe antioxidant peptide salamandrin-i: First bioactive peptide identified from skin secretion of salamandra genus (salamandra salamandra)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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