A multi-approach analysis highlights the relevance of RPA-1 as a telomere end-binding protein (TEBP) in Leishmania amazonensis

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorÉcole Normale Supérieure Paris-Saclay-
Autor(es): dc.contributorINSERM U1154-
Autor(es): dc.contributorUFR927-
Autor(es): dc.creatorFernandes, Carlos A.H. [UNESP]-
Autor(es): dc.creatorMorea, Edna Gicela O. [UNESP]-
Autor(es): dc.creatordos Santos, Gabriel A. [UNESP]-
Autor(es): dc.creatorda Silva, Vitor L. [UNESP]-
Autor(es): dc.creatorVieira, Marina Roveri [UNESP]-
Autor(es): dc.creatorViviescas, Maria Alejandra [UNESP]-
Autor(es): dc.creatorChatain, Jean-
Autor(es): dc.creatorVadel, Aurélie-
Autor(es): dc.creatorSaintomé, Carole-
Autor(es): dc.creatorFontes, Marcos Roberto M. [UNESP]-
Autor(es): dc.creatorCano, Maria Isabel Nogueira [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:29:51Z-
Data de disponibilização: dc.date.available2022-02-22T00:29:51Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bbagen.2020.129607-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200232-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200232-
Descrição: dc.descriptionBackground: Telomeres are chromosome end structures important in the maintenance of genome homeostasis. They are replenished by the action of telomerase and associated proteins, such as the OB (oligonucleotide/oligosaccharide-binding)-fold containing telomere-end binding proteins (TEBP) which plays an essential role in telomere maintenance and protection. The nature of TEBPs is well known in higher and some primitive eukaryotes, but it remains undetermined in trypanosomatids. Previous in silico searches have shown that there are no homologs of the classical TEPBs in trypanosomatids, including Leishmania sp. However, Replication Protein A subunit 1 (RPA-1), an OB-fold containing DNA-binding protein, was found co-localized with trypanosomatids telomeres and showed a high preference for the telomeric G-rich strand. Methods and results: We predicted the absence of structural homologs of OB-fold containing TEBPs in the Leishmania sp. genome using structural comparisons. We demonstrated by molecular docking that the ssDNA binding mode of LaRPA-1 shares features with the higher eukaryotes POT1 and RPA-1 crystal structures ssDNA binding mode. Using fluorescence spectroscopy, protein-DNA interaction assays, and FRET, we respectively show that LaRPA-1 shares some telomeric functions with the classical TEBPs since it can bind at least one telomeric repeat, protect the telomeric G-rich DNA from 3′-5′ Exonuclease I digestion, and unfold telomeric G-quadruplex. Conclusions: Our results suggest that RPA-1 emerges as a TEBP in trypanosomatids, and in this context, we present two possible evolutionary landscapes of trypanosomatids RPA-1 that could reflect upon the evolution of OB-fold containing TEBPs from all eukaryotes.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCentre National de la Recherche Scientifique-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionInstitut National de la Santé et de la Recherche Médicale-
Descrição: dc.descriptionMuséum National d'Histoire Naturelle-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Biosciences Institute São Paulo State University (UNESP) – Botucatu-
Descrição: dc.descriptionLaboratoire de Biologie et Pharmacologie Appliquée École Normale Supérieure Paris-Saclay-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences São Paulo State University (UNESP) – Botucatu-
Descrição: dc.descriptionMNHN CNRS UMR 7196 INSERM U1154, 43 rue Cuvier-
Descrição: dc.descriptionSorbonne Université UFR927, 4 place Jussieu-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Biosciences Institute São Paulo State University (UNESP) – Botucatu-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences São Paulo State University (UNESP) – Botucatu-
Descrição: dc.descriptionCAPES: 171895/2018-01-
Descrição: dc.descriptionFAPESP: 2015/17286-0-
Descrição: dc.descriptionFAPESP: 2015/18641-5-
Descrição: dc.descriptionFAPESP: 2018/0395-6-
Descrição: dc.descriptionFAPESP: 2018/04375-2-
Descrição: dc.descriptionFAPESP: 2019/11496-3-
Descrição: dc.descriptionCNPq: 305300/2006-7-
Descrição: dc.descriptionCAPES: 88881-
Idioma: dc.languageen-
Relação: dc.relationBiochimica et Biophysica Acta - General Subjects-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectG-quadruplex-
Palavras-chave: dc.subjectLeishmania sp.-
Palavras-chave: dc.subjectOB-fold-
Palavras-chave: dc.subjectRPA-1-
Palavras-chave: dc.subjectTelomere end-binding protein-
Título: dc.titleA multi-approach analysis highlights the relevance of RPA-1 as a telomere end-binding protein (TEBP) in Leishmania amazonensis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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