Great Abundance of Satellite DNA in Proceratophrys (Anura, Odontophrynidae) Revealed by Genome Sequencing

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorDa Silva, Marcelo J. [UNESP]-
Autor(es): dc.creatorFogarin Destro, Raquel [UNESP]-
Autor(es): dc.creatorGazoni, Thiago [UNESP]-
Autor(es): dc.creatorNarimatsu, Hideki [UNESP]-
Autor(es): dc.creatorPereira Dos Santos, Paulo S. [UNESP]-
Autor(es): dc.creatorHaddad, Célio F.B. [UNESP]-
Autor(es): dc.creatorParise-Maltempi, Patricia P. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:29:47Z-
Data de disponibilização: dc.date.available2022-02-22T00:29:47Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1159/000506531-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200210-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200210-
Descrição: dc.descriptionMost eukaryotic genomes contain substantial portions of repetitive DNA sequences. These are located primarily in highly compacted heterochromatin and, in many cases, are one of the most abundant components of the sex chromosomes. In this sense, the anuran Proceratophrys boiei represents an interesting model for analyses on repetitive sequences by means of cytogenetic techniques, since it has a karyotype with large blocks of heterochromatin and a ZZ/ZW sex chromosome system. The present study describes, for the first time, families of satellite DNA (satDNA) in the frog P. boiei. Its genome size was estimated at 1.6 Gb, of which 41% correspond to repetitive sequences, including satDNAs, rDNAs, transposable elements, and other elements characterized as non-repetitive. The satDNAs were mapped by FISH in the centromeric and pericentromeric regions of all chromosomes, suggesting a possible involvement of these sequences in centromere function. SatDNAs are also present in the W sex chromosome, occupying the entire heterochromatic area, indicating a probable contribution of this class of repetitive DNA to the differentiation of the sex chromosomes in this species. This study is a valuable contribution to the existing knowledge on repetitive sequences in amphibians. We show the presence of repetitive DNAs, especially satDNAs, in the genome of P. boiei that might be of relevance in genome organization and regulation, setting the stage for a deeper functional genome analysis of Proceratophrys.-
Descrição: dc.descriptionDepartamento de Biologia Instituto de Biociências Universidade Estadual Paulista - UNESP, Av. 24A, 1515-
Descrição: dc.descriptionDepartamento de Zoologia e Centro de Aquicultura Instituto de Biociências Universidade Estadual Paulista - UNESP-
Descrição: dc.descriptionDepartamento de Biologia Instituto de Biociências Universidade Estadual Paulista - UNESP, Av. 24A, 1515-
Descrição: dc.descriptionDepartamento de Zoologia e Centro de Aquicultura Instituto de Biociências Universidade Estadual Paulista - UNESP-
Formato: dc.format141-147-
Idioma: dc.languageen-
Relação: dc.relationCytogenetic and Genome Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAmphibia-
Palavras-chave: dc.subjectCytogenetics-
Palavras-chave: dc.subjectFluorescence in situ hybridization-
Palavras-chave: dc.subjectProceratophrys boiei-
Palavras-chave: dc.subjectRepeatExplorer-
Título: dc.titleGreat Abundance of Satellite DNA in Proceratophrys (Anura, Odontophrynidae) Revealed by Genome Sequencing-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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