Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorChico Mendes Institute of Biodiversity Conservation-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorFederal Rural University of Rio de Janeiro-
Autor(es): dc.creatorYasui, George Shigueki-
Autor(es): dc.creatorNakaghi, Laura Satiko Okada [UNESP]-
Autor(es): dc.creatorMonzani, Paulo Sérgio-
Autor(es): dc.creatorNascimento, Nivaldo Ferreira do [UNESP]-
Autor(es): dc.creatorPereira dos Santos, Matheus-
Autor(es): dc.creatorGoes, Caio Augusto Gomes [UNESP]-
Autor(es): dc.creatorPorto-Foresti, Fábio [UNESP]-
Autor(es): dc.creatorSenhorini, José Augusto-
Data de aceite: dc.date.accessioned2022-02-22T00:29:40Z-
Data de disponibilização: dc.date.available2022-02-22T00:29:40Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/jai.14025-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200176-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200176-
Descrição: dc.descriptionThe streaked prochilod Prochilodus lineatus is an important fish species from the Neotropical region. In this study, a protocol of triploidization was established by temperature shock. Fertilized eggs were shocked at 2 min post-fertilization at 0, 38, 40 and 42°C. At 0°C, the embryos were maintained for 30 min, while the rest for 2 min. Heat shocked embryos and control (during all experiment) were incubated at 27°C. The ploidy status was confirmed by flow cytometry, erythrocyte diameter, conventional cytogenetics (Giemsa staining), chromosome banding and fluorescence in situ hybridization using 5S and 18S rDNA probes. Heat-shock at 40°C produced 96.7% of triploids and such a procedure did not reduce the fertilization, hatching rate and the percentage of normal embryos. The use of chromosome banding and FISH gave rise to effective procedure to identify triploids. The data obtained here is innovative for the species and bring new information for basic and applied studies.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionLaboratory of Fish Biotechnology National Center for Research and Conservation of Continental Fish Chico Mendes Institute of Biodiversity Conservation-
Descrição: dc.descriptionDepartment of Veterinary Medicine – FZEA University of São Paulo-
Descrição: dc.descriptionAquaculture Center São Paulo State University-
Descrição: dc.descriptionFederal Rural University of Rio de Janeiro-
Descrição: dc.descriptionFaculdade de Ciências Bauru UNESP – Univ. Estadual Paulista-
Descrição: dc.descriptionAquaculture Center São Paulo State University-
Descrição: dc.descriptionFaculdade de Ciências Bauru UNESP – Univ. Estadual Paulista-
Descrição: dc.descriptionFAPESP: 2010/17429-1-
Descrição: dc.descriptionFAPESP: 2011/11664-1-
Formato: dc.format339-344-
Idioma: dc.languageen-
Relação: dc.relationJournal of Applied Ichthyology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcharacin-
Palavras-chave: dc.subjectchromosome-
Palavras-chave: dc.subjectfish-
Palavras-chave: dc.subjectpolyploid-
Título: dc.titleTriploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.