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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | University of Bonn | - |
Autor(es): dc.contributor | University Medical Center of the Johannes Gutenberg University | - |
Autor(es): dc.contributor | University of Bern | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Rath-Deschner, Birgit | - |
Autor(es): dc.creator | Memmert, Svenja | - |
Autor(es): dc.creator | Damanaki, Anna | - |
Autor(es): dc.creator | Nokhbehsaim, Marjan | - |
Autor(es): dc.creator | Eick, Sigrun | - |
Autor(es): dc.creator | Cirelli, Joni A. [UNESP] | - |
Autor(es): dc.creator | Götz, Werner | - |
Autor(es): dc.creator | Deschner, James | - |
Autor(es): dc.creator | Jäger, Andreas | - |
Autor(es): dc.creator | Nogueira, Andressa V. B. | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:29:30Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:29:30Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-10-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1007/s00784-020-03244-1 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/200130 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/200130 | - |
Descrição: dc.description | Objectives: This study was established to investigate whether the chemokines CXCL1, CCL2, and CCL5 are produced in periodontal cells and tissues and, if so, whether their levels are regulated by microbial and/or mechanical signals. Materials and methods: The chemokine expression and protein levels in gingival biopsies from patients with and without periodontitis were analyzed by RT-PCR and immunohistochemistry. The chemokines were also analyzed in gingival biopsies from rats subjected to experimental periodontitis and/or orthodontic tooth movement. Additionally, chemokine levels were determined in periodontal fibroblasts exposed to the periodontopathogen Fusobacterium nucleatum and mechanical forces by RT-PCR and ELISA. Results: Higher CXCL1, CCL2, and CCL5 levels were found in human and rat gingiva from sites of periodontitis as compared with periodontally healthy sites. In the rat experimental periodontitis model, the bacteria-induced upregulation of these chemokines was significantly counteracted by orthodontic forces. In vitro, F. nucleatum caused a significant upregulation of all chemokines at 1 day. When the cells were subjected simultaneously to F. nucleatum and mechanical forces, the upregulation of chemokines was significantly inhibited. The transcriptional findings were paralleled at protein level. Conclusions: This study provides original evidence in vitro and in vivo that the chemokines CXCL1, CCL2, and CCL5 are regulated by both microbial and mechanical signals in periodontal cells and tissues. Furthermore, our study revealed that biomechanical forces can counteract the stimulatory actions of F. nucleatum on these chemokines. Clinical relevance: Mechanical loading might aggravate periodontal infection by compromising the recruitment of immunoinflammatory cells. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Deutscher Akademischer Austauschdienst | - |
Descrição: dc.description | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | - |
Descrição: dc.description | Deutsche Forschungsgemeinschaft | - |
Descrição: dc.description | Department of Orthodontics Center of Dento-Maxillo-Facial Medicine University of Bonn, Welschnonnenstrasse 17 | - |
Descrição: dc.description | Section of Experimental Dento-Maxillo-Facial Medicine Center of Dento-Maxillo-Facial Medicine University of Bonn | - |
Descrição: dc.description | Department of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg University | - |
Descrição: dc.description | Department of Periodontology Laboratory for Oral Microbiology University of Bern | - |
Descrição: dc.description | Department of Diagnosis and Surgery School of Dentistry at Araraquara Sao Paulo State University UNESP | - |
Descrição: dc.description | Department of Diagnosis and Surgery School of Dentistry at Araraquara Sao Paulo State University UNESP | - |
Descrição: dc.description | FAPESP: 2014/20715-7 | - |
Descrição: dc.description | FAPESP: 2017/07137-2 | - |
Descrição: dc.description | Deutscher Akademischer Austauschdienst: 57391253 | - |
Descrição: dc.description | CAPES: 88881.144012/2017-01 | - |
Descrição: dc.description | Deutsche Forschungsgemeinschaft: DE1593/5-1 | - |
Formato: dc.format | 3661-3670 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Clinical Oral Investigations | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Fusobacterium nucleatum | - |
Palavras-chave: dc.subject | Gingivitis | - |
Palavras-chave: dc.subject | Orthodontic tooth movement | - |
Palavras-chave: dc.subject | Periodontitis | - |
Palavras-chave: dc.subject | Periodontium | - |
Título: dc.title | CXCL1, CCL2, and CCL5 modulation by microbial and biomechanical signals in periodontal cells and tissues—in vitro and in vivo studies | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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