Decreased excitability of locus coeruleus neurons during hypercapnia is exaggerated in the streptozotocin-model of Alzheimer's disease

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorA.T. Still University of Health Sciences-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorTruman State University-
Autor(es): dc.creatorVicente, Mariane C. [UNESP]-
Autor(es): dc.creatorHumphrey, Chuma M.-
Autor(es): dc.creatorGargaglioni, Luciane H. [UNESP]-
Autor(es): dc.creatorOstrowski, Tim D.-
Data de aceite: dc.date.accessioned2022-02-22T00:29:28Z-
Data de disponibilização: dc.date.available2022-02-22T00:29:28Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.expneurol.2020.113250-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200122-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200122-
Descrição: dc.descriptionThe locus coeruleus (LC) is a pontine nucleus important for respiratory control and central chemoreception. It is affected in Alzheimer's disease (AD) and alteration of LC cell function may account for respiratory problems observed in AD patients. In the current study, we tested the electrophysiological properties and CO2/pH sensitivity of LC neurons in a model for AD. Sporadic AD was induced in rats by intracerebroventricular injection of 2 mg/kg streptozotocin (STZ), which induces behavioral and molecular impairments found in AD. LC neurons were recorded using the patch clamp technique and tested for responses to CO2 (10% CO2, pH = 7.0). The majority (~60%) of noradrenergic LC neurons in adult rats were inhibited by CO2 exposure as indicated by a significant decrease in action potential (AP) discharge to step depolarizations. The STZ-AD rat model had a greater sensitivity to CO2 than controls. The increased CO2-sensitivity was demonstrated by a significantly stronger inhibition of activity during hypercapnia that was in part due to hyperpolarization of the resting membrane potential. Reduction of AP discharge in both groups was generally accompanied by lower LC network activity, depolarized AP threshold, increased AP repolarization, and increased current through a subpopulation of voltage-gated K+ channels (KV). The latter was indicated by enhanced transient KV currents particularly in the STZ-AD group. Interestingly, steady-state KV currents were reduced under hypercapnia, a change that would favor enhanced AP discharge. However, the collective response of most LC neurons in adult rats, and particularly those in the STZ-AD group, was inhibited by CO2.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Physiology Kirksville College of Osteopathic Medicine A.T. Still University of Health Sciences-
Descrição: dc.descriptionDepartment of Animal Morphology and Physiology Sao Paulo State University–UNESP/FCAV at Jaboticabal-
Descrição: dc.descriptionDepartment of Biology Truman State University-
Descrição: dc.descriptionDepartment of Animal Morphology and Physiology Sao Paulo State University–UNESP/FCAV at Jaboticabal-
Descrição: dc.descriptionFAPESP: 2017/21750-9-
Idioma: dc.languageen-
Relação: dc.relationExperimental Neurology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBrain slice-
Palavras-chave: dc.subjectBrainstem-
Palavras-chave: dc.subjectChemosensitivity-
Palavras-chave: dc.subjectCO2-
Palavras-chave: dc.subjectIntracerebroventricular-
Palavras-chave: dc.subjectNeurodegeneration-
Palavras-chave: dc.subjectPatch clamp-
Palavras-chave: dc.subjectPotassium currents-
Palavras-chave: dc.subjectSTZ-
Título: dc.titleDecreased excitability of locus coeruleus neurons during hypercapnia is exaggerated in the streptozotocin-model of Alzheimer's disease-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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