Transcriptional analysis of THP-1 cells infected with leishmania infantum indicates no activation of the inflammasome platform

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Mato Grosso do Sul (UFMS)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorGatto, Mariana [UNESP]-
Autor(es): dc.creatorBorim, Patrícia Aparecida [UNESP]-
Autor(es): dc.creatorWolf, Ivan Rodrigo [UNESP]-
Autor(es): dc.creatorda Cruz, Taís Fukuta [UNESP]-
Autor(es): dc.creatorMota, Gustavo Augusto Ferreira [UNESP]-
Autor(es): dc.creatorBraz, Aline Márcia Marques [UNESP]-
Autor(es): dc.creatorAmorim, Bárbara Casella [UNESP]-
Autor(es): dc.creatorValente, Guilherme Targino [UNESP]-
Autor(es): dc.creatorGolim, Marjorie de Assis [UNESP]-
Autor(es): dc.creatorVenturini, James-
Autor(es): dc.creatorJunior, João Pessoa Araújo [UNESP]-
Autor(es): dc.creatorPontillo, Alessandra-
Autor(es): dc.creatorSartori, Alexandrina [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:29:08Z-
Data de disponibilização: dc.date.available2022-02-22T00:29:08Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1371/journal.pntd.0007949-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200025-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200025-
Descrição: dc.descriptionLeishmaniasis is caused by intracellular parasites transmitted to vertebrates by sandfly bites. Clinical manifestations include cutaneous, mucosal or visceral involvement depending upon the host immune response and the parasite species. To assure their survival inside macro-phages, these parasites developed a plethora of highly successful strategies to manipulate various immune system pathways. Considering that inflammasome activation is critical for the establishment of a protective immune response in many parasite infections, in this study we determined the transcriptome of THP-1 cells after infection with L. infantum, with a particular focus on the inflammasome components. To this end, the human cell line THP-1, previously differentiated into macrophages by PMA treatment, was infected with L. infantum promasti-gotes. Differentiated THP-1 cells were also stimulated with LPS to be used as a comparative parameter. The gene expression signature was determined 8 hours after by RNA-seq tech-nique. Infected or uninfected THP-1 cells were stimulated with nigericin (NIG) to measure active caspase-1 and TNF-α, IL-6 and IL-1β levels in culture supernatants after 8, 24 and 48 hours. L. infantum triggered a gene expression pattern more similar to non-infected THP-1 cells and very distinct from LPS-stimulated cells. Some of the most up-regulated genes in L. infantum-infected cells were CDC20, CSF1, RPS6KA1, CD36, DUSP2, DUSP5, DUSP7 and TNFAIP3. Some up-regulated GO terms in infected cells included cell coagulation, regulation of MAPK cascade, response to peptide hormone stimulus, negative regulation of transcription from RNA polymerase II promoter and nerve growth factor receptor signaling pathway. Infection was not able to induce the expression of genes associated with the inflammasome signaling pathway. This finding was confirmed by the absence of caspase-1 activation and IL-1β production after 8, 24 and 48 hours of infection. Our results indicate that L. infantum was unable to activate the inflammasomes during the initial interaction with THP-1 cells.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionTropical Diseases Department Botucatu Medical School – UNESP-
Descrição: dc.descriptionBioprocess and Biotechnology Department Agronomic Sciences School – UNESP-
Descrição: dc.descriptionMicrobiology and Immunology Department Biosciences Institute-UNESP-
Descrição: dc.descriptionInternal Medicine Department Botucatu Medical School – UNESP-
Descrição: dc.descriptionFlow Cytometry Laboratory Botucatu Medical School-UNESP-
Descrição: dc.descriptionMedical School – UFMS-
Descrição: dc.descriptionImmunology Department São Paulo University – USP-
Descrição: dc.descriptionTropical Diseases Department Botucatu Medical School – UNESP-
Descrição: dc.descriptionBioprocess and Biotechnology Department Agronomic Sciences School – UNESP-
Descrição: dc.descriptionMicrobiology and Immunology Department Biosciences Institute-UNESP-
Descrição: dc.descriptionInternal Medicine Department Botucatu Medical School – UNESP-
Descrição: dc.descriptionFlow Cytometry Laboratory Botucatu Medical School-UNESP-
Descrição: dc.descriptionFAPESP: 2014/04019-0-
Formato: dc.format1-24-
Idioma: dc.languageen-
Relação: dc.relationPLoS Neglected Tropical Diseases-
???dc.source???: dc.sourceScopus-
Título: dc.titleTranscriptional analysis of THP-1 cells infected with leishmania infantum indicates no activation of the inflammasome platform-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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