Simple and Rapid Method by Ultra High-Performance Liquid Chromatography (UHPLC) with Ultraviolet Detection for Determination of Efavirenz in Plasma: Application in a Preclinical Pharmacokinetic Study

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorDos Santos Martins, Evelin [UNESP]-
Autor(es): dc.creatorOliveira, Jonata Augusto [UNESP]-
Autor(es): dc.creatorFranchin, Taísa Busaranho [UNESP]-
Autor(es): dc.creatorSilva, Bruna Cristina Ulian [UNESP]-
Autor(es): dc.creatorCândido, Caroline Damico [UNESP]-
Autor(es): dc.creatorPeccinini, Rosângela Gonçalves [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:29:03Z-
Data de disponibilização: dc.date.available2022-02-22T00:29:03Z-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2020-12-11-
Data de envio: dc.date.issued2019-10-10-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/chromsci/bmz078-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/200000-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/200000-
Descrição: dc.descriptionA simple and rapid ultra-high-performance liquid chromatography (UHPLC) method for determination of efavirenz (EFV) in plasma was developed and applied in a preclinical pharmacokinetic study. The method involves only addition of acetonitrile to precipitation of plasma proteins followed by solvent evaporation. The mobile phase consisted of methanol, acetonitrile and 0.1 M formic acid (20:50:30) at a flow rate of 0.3 mL/min with run time of 5 min. A CSH C18 column and a UHPLC-UV system operating at 245 nm were used. There was a linear response in the range of 0.078 to 10 μg/mL, and the equation was obtained by weighting (1/x2) with r2 = 0.9965. The pharmacokinetic disposition of EFV was investigated in rabbits (two groups, n = 7) following a single intravenous administration (IV group) at a dose of 2.7 mg/kg and a single oral administration (oral group) of EFV co-administered with lamivudine (3TC) and tenofovir (TNF) at a dose of 50, 25 and 25 mg, respectively. The study demonstrated the applicability of the method for determination of EFV in plasma without interference from other co-administered drugs, and the pharmacokinetic parameters were calculated. The method showed advantages over other methods in the literature, such as simplicity of sample processing and fast results.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Natural Active Principles and Toxicology School of Pharmaceutical Sciences Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP-
Descrição: dc.descriptionDepartment of Natural Active Principles and Toxicology School of Pharmaceutical Sciences Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP-
Formato: dc.format874-880-
Idioma: dc.languageen-
Relação: dc.relationJournal of Chromatographic Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAIDS-
Palavras-chave: dc.subjectBioanalytical method-
Palavras-chave: dc.subjectEfavirenz-
Palavras-chave: dc.subjectPharmacokinetics-
Palavras-chave: dc.subjectUHPLC-
Título: dc.titleSimple and Rapid Method by Ultra High-Performance Liquid Chromatography (UHPLC) with Ultraviolet Detection for Determination of Efavirenz in Plasma: Application in a Preclinical Pharmacokinetic Study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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