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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Dos Santos Martins, Evelin [UNESP] | - |
Autor(es): dc.creator | Oliveira, Jonata Augusto [UNESP] | - |
Autor(es): dc.creator | Franchin, Taísa Busaranho [UNESP] | - |
Autor(es): dc.creator | Silva, Bruna Cristina Ulian [UNESP] | - |
Autor(es): dc.creator | Cândido, Caroline Damico [UNESP] | - |
Autor(es): dc.creator | Peccinini, Rosângela Gonçalves [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:29:03Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:29:03Z | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2020-12-11 | - |
Data de envio: dc.date.issued | 2019-10-10 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1093/chromsci/bmz078 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/200000 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/200000 | - |
Descrição: dc.description | A simple and rapid ultra-high-performance liquid chromatography (UHPLC) method for determination of efavirenz (EFV) in plasma was developed and applied in a preclinical pharmacokinetic study. The method involves only addition of acetonitrile to precipitation of plasma proteins followed by solvent evaporation. The mobile phase consisted of methanol, acetonitrile and 0.1 M formic acid (20:50:30) at a flow rate of 0.3 mL/min with run time of 5 min. A CSH C18 column and a UHPLC-UV system operating at 245 nm were used. There was a linear response in the range of 0.078 to 10 μg/mL, and the equation was obtained by weighting (1/x2) with r2 = 0.9965. The pharmacokinetic disposition of EFV was investigated in rabbits (two groups, n = 7) following a single intravenous administration (IV group) at a dose of 2.7 mg/kg and a single oral administration (oral group) of EFV co-administered with lamivudine (3TC) and tenofovir (TNF) at a dose of 50, 25 and 25 mg, respectively. The study demonstrated the applicability of the method for determination of EFV in plasma without interference from other co-administered drugs, and the pharmacokinetic parameters were calculated. The method showed advantages over other methods in the literature, such as simplicity of sample processing and fast results. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Department of Natural Active Principles and Toxicology School of Pharmaceutical Sciences Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP | - |
Descrição: dc.description | Department of Natural Active Principles and Toxicology School of Pharmaceutical Sciences Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP | - |
Formato: dc.format | 874-880 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Journal of Chromatographic Science | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | AIDS | - |
Palavras-chave: dc.subject | Bioanalytical method | - |
Palavras-chave: dc.subject | Efavirenz | - |
Palavras-chave: dc.subject | Pharmacokinetics | - |
Palavras-chave: dc.subject | UHPLC | - |
Título: dc.title | Simple and Rapid Method by Ultra High-Performance Liquid Chromatography (UHPLC) with Ultraviolet Detection for Determination of Efavirenz in Plasma: Application in a Preclinical Pharmacokinetic Study | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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